Proteintech

EPHA4 Polyclonal Antibody

Product Image Gallery

EPHA4 Antibody in Immunofluorescence (IF) — IF result of EPHA4 antibody (21875-1-AP, 1:50) with culture neuron cells by Wilson Pak Kin Lou.

EPHA4 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21875-1-AP (EPHA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EPHA4 Antibody in Flow Cytometry (Flow) — 1X10^6 SH-SY5Y cells were stained with 0.2ug EPHA4 antibody (21875-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

EPHA4 Antibody in Immunoprecipitation (IP) — IP result of anti-EPHA4 (IP:21875-1-AP, 4ug; Detection:21875-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

Product Specifications

Species

Human, Mouse

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EPHA4 Fusion Protein Ag16233

Conjugate

Unconjugated

Form

Liquid

Concentration

0.46 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

These proteins belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EK8; epa4; EPH-like kinase 8; Ephrin type-A receptor 4; receptor protein-tyrosine kinase HEK8; TYRO1 protein tyrosine kinase; Tyrosine-protein kinase receptor MPK-3; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase receptor SEK-1; Tyrosine-protein kinase TYRO1

Gene Aliases: 2900005C20Rik; AI385584; Cek8; EPHA4; HEK8; rb; SEK; Sek1; TYRO1

UniProt ID: (Human) P54764, (Mouse) Q03137

Entrez Gene ID: (Human) 2043, (Mouse) 13838

Function(s)