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ERK1 C-Term Polyclonal Antibody
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FIGURE: 1 / 3
ERK1 C-Term Antibody (600-401-GP9S) in WB
Western Blot of Rabbit Anti-ERK1 C-Term Antibody. Lane 1:MW Ladder (Opal Pre-Stained). Lane 2:HEK293 WCL. Lane 3: HeLa WCL. Lane 4: MCF-7 WCL. Lane 5: Jurkat WCL. Lane 6: A431 WCL. Lane 7: A549 WCL. Lane 8: LNCaP WCL. Lane 9: MOLT-4 WCL. Lane 10: Ramos WCL. Lane 11: Raji WCL. Lane 12: A-172 WCL. Lane 13: NIH/3T3 WCL. Lane 14: MW Ladder. Load: 10ng. Blocking: 1% Casein/TTBS buffer. Primary Antibody: ERK1 at 1µg/mL in 1% Casein/TTBS overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (611-103-122) 1:30,000 in 1% Casein/TTBS for 1 hr a... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
600-401-GP9S
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
25 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
This affinity purified antibody is directed against human ERK1 protein. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that is an important activator of p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Functionally, ERK1 is involved with a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. ERK1 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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