|Tested species reactivity||Rat, Non-human primate, Dog, Guinea pig, Hamster, Human, Bovine, Mouse, Rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: (C)FIDEHATKRSRTKEEL, with Cysteine added, conjugated to KLH, corresponding to amino acids 623-638 of Mouse ERp72|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20797 detects ERp72 in human, mouse, rat, bovine, canine, guinea pig, hamster, non-human primate, and rabbit samples.
PA1-20797 has been successfully used in Western blot procedures.
The PA1-20797 immunogen is a synthetic peptide, KLH conjugated, corresponding to residues C-F(623 ) I D E H A T K R S R T K E E L(638) of mouse ERp72.
ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.