|Tested species reactivity||Human|
|Published species reactivity||Xenopus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of ETV1 (aa1-191) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
MA5-15461 targets ETV1 in WB applications and shows reactivity with Human samples.
The MA5-15461 immunogen is purified recombinant fragment of ETV1 (aa1-191) expressed in E. Coli.
ETV1: ets variant gene 1. Several members of the Ets gene family encode sequence-specific DNA binding proteins that recognize DNA sequences with a centrally located 5'-GGAA-3' element. All of the Ets proteins recognize the same central core sequence but each protein interacts with unique sequences that flank this core. ER81 (also designated ETV1) binds the motif 5'-CGGAA/T-3'. ER81 is highly expressed in brain, testis, lung and heart. ER81 is also moderately expressed in spleen, pancreas, colon and small intestine. During development, ER81 displays a unique expression patterns which suggests that this transcriptional factor might play an important role in organogenesis. ERK-1 activates ER81 transcriptional activity while MAPKAP kinase 2 inhibits ER81.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Plakophilin-3 catenin associates with the ETV1/ER81 transcription factor to positively modulate gene activity.
MA5-15461 was used in immunoprecipitation to study the regulation of gene activity via the interaction of plakophilin-3 with the ETV1/ER81 transcription factor
|Munoz WA,Lee M,Miller RK,Ahmed Z,Ji H,Link TM,Lee GR,Kloc M,Ladbury JE,McCrea PD||PloS one (9:null)||2014|