|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COOH-terminal amino acids of mouse ETB (424–442) KANDHGYDNFRSSNKYSSS coupled to KLH|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:2000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2000|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA3-066 detects Endothelin Receptor B from human, mouse, and rat samples.
PA3-066 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects a 47 kDa protein corresponding to mouse Endothelin Receptor B.
The PA3-066 antigen is the COOH-terminal amino acids of mouse ETB (424–442) KANDHGYDNFRSSNKYSSS coupled to KLH.
The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Three transcript variants encoding two different isoforms have been found for this gene. While both isoforms bind ET1, they exhibit different responses upon binding, suggesting that they may be functionally distinct.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Transforming growth factor-ß regulates endothelin-1 signaling in the newborn mouse lung during hypoxia exposure.
PA3-066 was used in western blot to investigate the influence of tumor growth factor beta on endothelin-1 expression and effects of hypoxia on the newborn lung
|Olave N,Nicola T,Zhang W,Bulger A,James M,Oparil S,Chen YF,Ambalavanan N||American journal of physiology. Lung cellular and molecular physiology (302:L857)||2012|