Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of Epithelial Specific Antigen (green) showing staining in the membrane of HT29 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Epithelial Specific Antigen monoclonal antibody (Product # MA5-12436) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MCF-7 human breast cancer cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Radioimmune Assays (RIA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12436 targets Epithelial Specific Antigen in FACS, ICC/IF, IHC (P), and RIA applications and shows reactivity with Human and Mouse samples.
The MA5-12436 immunogen is mCF-7 human breast cancer cells.
EGP40 is a 40kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. It reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.
MA5-12436 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells
|Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK||Anticancer research (33:4875)||2013|
Genetic vaccines against Ep-CAM break tolerance to self in a limited subset of subjects: initial identification of predictive biomarkers.
MA5-12436 was used in flow cytometry to evaluate the Ep-CAM genetic vaccination strategy in animal models
|Elia L,Mennuni C,Storto M,Podda S,Calvaruso F,Salucci V,Aurisicchio L,Scarito A,Ciliberto G,La Monica N,Palombo F||European journal of immunology (36:1337)||2006|
Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept.
MA5-12436 was used in immunocytochemistry to develop a process involving leukapheresis, elutriation and FACS to isolate circulating tumor cells from large volumes
|Eifler RL,Lind J,Falkenhagen D,Weber V,Fischer MB,Zeillinger R||Cytometry. Part B, Clinical cytometry (80:100)||2011|
Inhibition of proliferation by PERK regulates mammary acinar morphogenesis and tumor formation.
MA5-12436 was used in immunocytochemistry to study the role of adhesion-dependent regulation of PERK activity in modulating mammary acinar morphogenesis and breast tumorigenesis via inhibition of proliferation
|Sequeira SJ,Ranganathan AC,Adam AP,Iglesias BV,Farias EF,Aguirre-Ghiso JA||PloS one (2:null)||2007|
Overexpression of epithelial cell adhesion molecule in primary, metastatic, and recurrent/chemotherapy-resistant epithelial ovarian cancer: implications for epithelial cell adhesion molecule-specific immunotherapy.
MA5-12436 was used in immunohistochemistry to study the therapeutic implications of epithelial cell adhesion molecule overexpression in epithelial ovarian carcinomas
|Bellone S,Siegel ER,Cocco E,Cargnelutti M,Silasi DA,Azodi M,Schwartz PE,Rutherford TJ,Pecorelli S,Santin AD||International journal of gynecological cancer : official journal of the International Gynecological Cancer Society (19:860)||2009|
EpCAM-specific vaccine response by modified antigen and chimeric costimulatory molecule in cynomolgus monkeys.
MA5-12436 was used in western blot to examine a novel costimulatory adjuvant for tumor immuno therapy
|Neighbors M,Apt D,Chang JC,Brinkman A,Sipos-Solman I,Ong R,Leong S,Punnonen J||Journal of immunotherapy (Hagerstown, Md. : 1997) (31:644)||2008|
adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP40; EPCAM; epithelial cell adhesion molecule; epithelial glycoprotein 314; Epithelial Specific Antigen; ESA; human epithelial glycoprotein-2; KS1/4; lymphocyte antigen 74; M4S1; major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; panepithelial glycoprotein 314; protein 289A; TACSTD1; Trop-1 protein; TROP1; tumor-associated calcium signal transducer 1
CD326; DIAR5; EGP; EGP-2; EGP314; EGP40; Ep-CAM; EPCAM; EpCAM1; ESA; GA733-2; gp40; HNPCC8; KS1/4; KSA; Ly74; M1S2; M4S1; MIC18; MK-1; Tacsd1; TACSTD1; TROP1