Immunofluorescent analysis of Epithelial Specific Antigen (green) showing staining in the membrane of HT29 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Epithelial Specific Antigen monoclonal antibody (Product # MA5-12436) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MCF-7 human breast cancer cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Radioimmune Assays (RIA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12436 targets Epithelial Specific Antigen in FACS, ICC/IF, IHC (P), and RIA applications and shows reactivity with Human and Mouse samples.
The MA5-12436 immunogen is mCF-7 human breast cancer cells.
EGP40 is a 40kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. It reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Interpolation method for accurate affinity ranking of arrayed ligand-analyte interactions.
MA5-12436 was used to study accurate affinity ranking of arrayed ligand-analyte interactions by an interpolation method
|Schasfoort RB,Andree KC,van der Velde N,van der Kooi A,Stojanovi¿ I,Terstappen LW||Analytical biochemistry (500:21)||2016|
Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.
MA5-12436 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells
|Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK||Anticancer research (33:4875)||2013|
Genetic vaccines against Ep-CAM break tolerance to self in a limited subset of subjects: initial identification of predictive biomarkers.
MA5-12436 was used in flow cytometry to evaluate the Ep-CAM genetic vaccination strategy in animal models
|Elia L,Mennuni C,Storto M,Podda S,Calvaruso F,Salucci V,Aurisicchio L,Scarito A,Ciliberto G,La Monica N,Palombo F||European journal of immunology (36:1337)||2006|
Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept.
MA5-12436 was used in immunocytochemistry to develop a process involving leukapheresis, elutriation and FACS to isolate circulating tumor cells from large volumes
|Eifler RL,Lind J,Falkenhagen D,Weber V,Fischer MB,Zeillinger R||Cytometry. Part B, Clinical cytometry (80:100)||2011|
Inhibition of proliferation by PERK regulates mammary acinar morphogenesis and tumor formation.
MA5-12436 was used in immunocytochemistry to study the role of adhesion-dependent regulation of PERK activity in modulating mammary acinar morphogenesis and breast tumorigenesis via inhibition of proliferation
|Sequeira SJ,Ranganathan AC,Adam AP,Iglesias BV,Farias EF,Aguirre-Ghiso JA||PloS one (2:null)||2007|
Overexpression of epithelial cell adhesion molecule in primary, metastatic, and recurrent/chemotherapy-resistant epithelial ovarian cancer: implications for epithelial cell adhesion molecule-specific immunotherapy.
MA5-12436 was used in immunohistochemistry to study the therapeutic implications of epithelial cell adhesion molecule overexpression in epithelial ovarian carcinomas
|Bellone S,Siegel ER,Cocco E,Cargnelutti M,Silasi DA,Azodi M,Schwartz PE,Rutherford TJ,Pecorelli S,Santin AD||International journal of gynecological cancer : official journal of the International Gynecological Cancer Society (19:860)||2009|
EpCAM-specific vaccine response by modified antigen and chimeric costimulatory molecule in cynomolgus monkeys.
MA5-12436 was used in western blot to examine a novel costimulatory adjuvant for tumor immuno therapy
|Neighbors M,Apt D,Chang JC,Brinkman A,Sipos-Solman I,Ong R,Leong S,Punnonen J||Journal of immunotherapy (Hagerstown, Md. : 1997) (31:644)||2008|