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Immunohistochemistry analysis of EpCAM / CD326 (MOC-31) showing staining in the membrane and weak cytoplasm of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a EpCAM / CD326 Antibody (MOC-31) Mouse Monoclonal Antibody (MA512442) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Neuraminidase treated cells from a variant small cell lung carcinoma cell line (GLS-1)|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12442 targets Epithelial Specific Antigen in IHC (P) applications and shows reactivity with Human samples.
The MA5-12442 immunogen is neuraminidase treated cells from a variant small cell lung carcinoma cell line (GLS-1).
Epithelial Specific Antigen/Ep-CAM
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP40; EPCAM; epithelial glycoprotein 314; Epithelial Specific Antigen; ESA; human epithelial glycoprotein-2; KS1/4; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; tumor-associated calcium signal transducer 1
DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1