Invitrogen

EphB2 Monoclonal Antibody (1A6C9)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

16 References

View all (24) EphB2 antibodies

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EphB2 Antibody in Western Blot (WB) — Western blot was performed using Anti-EphB2 Monoclonal Antibody (1A6C9) (Product # 37-1700) and 117, 110kDa bands corresponding to EphB2 was increased upon VEGF treatment in HUVEC. Also, the expression was highest in COLO 205 in comparison to SW480 and HT-29 as reported. Membrane enriched cell extracts (30 ug lysate) of HUVEC (Lane 1), HUVEC treated with VEGF (20ng/ml, 10 min) (Lane 2), COLO 205 (Lane 3), SW480 (Lane 4) and HT-29 (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

EphB2 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP) — Figure 6 EphB receptors Induce Tyrosine Phosphorylation of NrCAM at the FIGQY Motif, and Inhibit Ankyrin Recruitment in HEK293 cells. A. NrCAM was expressed with or without EphB2 or EphB2 kinase dead mutant (EphB2 KD) in transfected HEK293 cells, followed by immunoprecipitation with NrCAM antibodies and immunoblotting with phospho-FIGQY antibodies. EphB2 induced phosphorylation of NrCAM (200 kD) at FIGQY (p-FIGQY) compared to NrCAM alone or to co-expression of the EphB2 kinase dead mutant. Blots were stripped and reprobed with antibodies to NrCAM to indicate relative levels of NrCAM in the immunoprecipitates. B. NrCAM was expressed with or without EphB1, EphB2, or EphB3 in transfected HEK293 cells, followed by immunoprecipitation with NrCAM antibodies and immunoblotting with phospho-FIGQY antibodies. EphB1 (HA-tagged) or EphB2 (not HA-tagged) induced effective phosphorylation of NrCAM at FIGQY, whereas EphB3 (HA-tagged) had a weak effect. Blots were stripped and reprobed with antibodies to EphB2 or to the HA-epitope on EphB1 and EphB3. Each EphB receptor was co-immunoprecipitated efficiently with NrCAM. C. Lysates of the superior colliculus from WT, EphB1/3 double null, EphB1/2/3 triple null and constitutively active EphB2 (F620D) homozygous mice (P2-P3) were immunoprecipitated with antibodies to NrCAM, and immunoblotted with phospho-FIGQY or NrCAM antibodies. Bands were quantified using the threshold function of ImageJ. The numbers under the blots represent the normalized av

EphB2 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Figure 1. Multiple guidance cue receptors interact with ribosomes. ( A ) Volcano plots showing statistically enriched proteins in DCC-IP and Nrp1-IP samples identified by permutation-based FDR-corrected t-test based on three biological replicates. The LFQ intensity of the DCC or Nrp1 pulldowns over IgG pulldowns are plotted against the -log10 p-value. FDR < 0.05; S0 = 2. ( B ) Gene enrichment analysis of statistically enriched proteins in the DCC and Nrp1 pulldown samples. The values in each circle denotes protein count. ( C-F ) Western blot validation of RP co-immunoprecipitation with DCC, Nrp1 and Robo2 but not with EphB2. Each Western blot was repeated 2 to 4 times, representative images are shown. ( G-J ) Relative 18S and 28S ribosomal RNA abundance after control (IgG) pulldown or receptors pulldowns shows enrichment of rRNA in DCC, Nrp1, and Robo2 but not EphB2 pulldowns (unpaired two-tailed t-test; three biological replicates). Bars indicate means, error bars indicate standard deviation; *p<0.05. Figure 1--figure supplement 1. Multiple guidance cue receptors interact with ribosomes in SH-SY5Y cells. ( A-C ) Western blot validation of RP co-immunoprecipitation with DCC, Nrp1 and Robo2 in SH-SY5Y cells. Western blots were repeated 2 to 4 times, Rps4X Western blots are from one experiment, representative examples are shown. ( D-E ) Relative 18S and 28S ribosomal RNA abundance after control (IgG) pulldowns or receptor pulldowns shows enrichment of rRNA in DCC and Nrp1 IPs i

EphB2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of EphB2 was observed in COLO 205 in comparison to SW480 and HT-29 using EphB2 Monoclonal Antibody (1A6C9) (Product # 37-1700) in western blot. {RE}

Product Details

37-1700

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	View 9 publications 9 publications
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
ELISA (ELISA)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	View 3 publications 3 publications
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Chicken, Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	1A6C9
Immunogen	A synthetic peptide derived from the C-terminal region of the recombinant EphB2 protein.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533302

Target Information

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A T cell redirection platform for co-targeting dual antigens on solid tumors.

mAbs

Enderle L,Shalaby KH,Gorelik M,Weiss A,Blazer LL,Paduch M,Cardarelli L,Kossiakoff A,Adams JJ,Sidhu SS

37-1700 was used in Western Blot to engineer the Dual Antigen T cell Engager to enable T cell activity to specific solid cancer cell features.

Tue Dec 28 00:00:00 EST 2021

The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons.

The Journal of biological chemistry

Bodin R,Paillé V,Oullier T,Durand T,Aubert P,Le Berre-Scoul C,Hulin P,Neunlist M,Cissé M

Published figure using EphB2 monoclonal antibody (Product # 37-1700) in Western Blot

Mon Nov 01 00:00:00 EDT 2021

Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility.

Nature communications

Washburn HR,Xia NL,Zhou W,Mao YT,Dalva MB

37-1700 was used in Western Blot to demonstrate that the positive surface charge of the hinge region of the N-terminal domain in the GluN1 subunit of the NMDAR is required to maintain NMDARs at dendritic spine synapses and mediates the direct extracellular interaction with a negatively charged phospho-tyrosine on the receptor tyrosine kinase EphB2.

Wed Jan 29 00:00:00 EST 2020

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

eLife

Koppers M,Cagnetta R,Shigeoka T,Wunderlich LC,Vallejo-Ramirez P,Qiaojin Lin J,Zhao S,Jakobs MA,Dwivedy A,Minett MS,Bellon A,Kaminski CF,Harris WA,Flanagan JG,Holt CE

Published figure using EphB2 monoclonal antibody (Product # 37-1700) in Immunoprecipitation

Wed Nov 20 00:00:00 EST 2019

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

eLife

Koppers M,Cagnetta R,Shigeoka T,Wunderlich LC,Vallejo-Ramirez P,Qiaojin Lin J,Zhao S,Jakobs MA,Dwivedy A,Minett MS,Bellon A,Kaminski CF,Harris WA,Flanagan JG,Holt CE

Published figure using EphB2 monoclonal antibody (Product # 37-1700) in Immunoprecipitation

Wed Nov 20 00:00:00 EST 2019

Role of forward and reverse signaling in Eph receptor and ephrin mediated cell segregation.

Experimental cell research

Wu Z,Ashlin TG,Xu Q,Wilkinson DG

37-1700 was used in Western Blot to propose that although polarised forward signaling is the principal driver of segregation, reverse signaling enables bidirectional repulsion which prevents mingling of each population into the other.

Thu Aug 01 00:00:00 EDT 2019

Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2.

Molecular biology of the cell

Okumura F,Joo-Okumura A,Obara K,Petersen A,Nishikimi A,Fukui Y,Nakatsukasa K,Kamura T

Published figure using EphB2 monoclonal antibody (Product # 37-1700) in Western Blot

Wed Nov 15 00:00:00 EST 2017

YES oncogenic activity is specified by its SH4 domain and regulates RAS/MAPK signaling in colon carcinoma cells.

American journal of cancer research

Dubois F,Leroy C,Simon V,Benistant C,Roche S

37-1700 was used in immunoprecipitation to elucidate how YES contributes to colorectal cancer progression

Thu Aug 13 00:00:00 EDT 2015

The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa.

The Journal of biological chemistry

Eriksson O,Ramström M,Hörnaeus K,Bergquist J,Mokhtari D,Siegbahn A

37-1700 was used in western blot to identify Eph tyrosine kinase receptors as the substrates of tissue factor/coagulation factor VIIa

Fri Nov 21 00:00:00 EST 2014

The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa.

The Journal of biological chemistry

Eriksson O,Ramström M,Hörnaeus K,Bergquist J,Mokhtari D,Siegbahn A

37-1700 was used in western blot to identify Eph tyrosine kinase receptors as the substrates of tissue factor/coagulation factor VIIa

Fri Nov 21 00:00:00 EST 2014

The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa.

The Journal of biological chemistry

Eriksson O,Ramström M,Hörnaeus K,Bergquist J,Mokhtari D,Siegbahn A

37-1700 was used in western blot to identify Eph tyrosine kinase receptors as the substrates of tissue factor/coagulation factor VIIa

Fri Nov 21 00:00:00 EST 2014

PloS one

Dai J,Buhusi M,Demyanenko GP,Brennaman LH,Hruska M,Dalva MB,Maness PF

37-1700 was used in western blot to assess the role of neuron-glial related cell adhesion molecule in retinotopic mapping.

Wed Apr 02 00:00:00 EDT 2014

PloS one

Dai J,Buhusi M,Demyanenko GP,Brennaman LH,Hruska M,Dalva MB,Maness PF

37-1700 was used in western blot to assess the role of neuron-glial related cell adhesion molecule in retinotopic mapping.

Wed Apr 02 00:00:00 EDT 2014

PloS one

Dai J,Buhusi M,Demyanenko GP,Brennaman LH,Hruska M,Dalva MB,Maness PF

37-1700 was used in western blot to assess the role of neuron-glial related cell adhesion molecule in retinotopic mapping.

Wed Apr 02 00:00:00 EDT 2014

The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases.