Invitrogen

ErbB2 (HER-2) Monoclonal Antibody (EP1045Y)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

6 References

View all (272) Her-2 (ErbB2) antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Antibody Testing Data (5)
Advanced Verification (2)

ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

ErbB2 (HER-2) Antibody (MA5-14488) in ICC/IF

Immunofluorescence analysis of Her2 was performed using 70% confluent log phase SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Her2 Rabbit monoclonal Antibody (Product # MA5-14488) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 de... View More

ErbB2 (HER-2) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ErbB2 (HER-2) Antibody in Western Blot (WB)

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Product Details

MA5-14488

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunocytochemistry (ICC/IF)

1:250

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

EP1045Y

Immunogen

A synthetic peptide corresponding to the c-terminus of human Her2

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_11006228

Product Specific Information

MA5-14488 targets HER-2 in IHC (P, F) applications and shows reactivity with Human samples.

The MA5-14488 immunogen is a synthetic peptide corresponding to the c-terminus of human Her2.

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The clinicopathological and prognostic significance of CD24, CD44, CD133, ALDH1 expressions in invasive ductal carcinoma of the breast: CD44/CD24 expression in breast cancer.

Pathology, research and practice

Kapucuoğlu N,Bozkurt KK,Başpınar Ş,Koçer M,Eroğlu HE,Akdeniz R,Akçil M

MA5-14488 was used in immunohistochemistry to evaluate the expression of CD24, CD44, CD133 and ALDH1 in invasive ductal carcinoma of the breast

Thu Oct 01 00:00:00 EDT 2015

Quantitation of fixative-induced morphologic and antigenic variation in mouse and human breast cancers.

Laboratory investigation; a journal of technical methods and pathology

Cardiff RD,Hubbard NE,Engelberg JA,Munn RJ,Miller CH,Walls JE,Chen JQ,Velásquez-García HA,Galvez JJ,Bell KJ,Beckett LA,Li YJ,Borowsky AD

MA5-14488 was used in immunohistochemistry to study the effect of different classes of fixative on the morphology and immunoreactivity of murine anfd human breast cancer specimens using quantitative image analysis

Mon Apr 01 00:00:00 EDT 2013

STAT3, a Poor Survival Predicator, Is Associated with Lymph Node Metastasis from Breast Cancer.

Journal of breast cancer

Chen Y,Wang J,Wang X,Liu X,Li H,Lv Q,Zhu J,Wei B,Tang Y

MA5-14488 was used in immunohistochemistry to study the mechanism by which STAT3 promotes the progression of breast cancer and its utility as a predictor of overall survival

Fri Mar 01 00:00:00 EST 2013

Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma.

Scientific reports

Qiu SQ,Wei XL,Huang WH,Wu MY,Qin YS,Li YK,Zhang GJ

MA5-14488 was used in immunohistochemistry - paraffin section to report the clinicopathological features of 9 breast malignant fibrous histiocytoma patients

Fri Feb 14 00:00:00 EST 2014

Combinatorial treatment of mammospheres with trastuzumab and salinomycin efficiently targets HER2-positive cancer cells and cancer stem cells.

International journal of cancer

Oak PS,Kopp F,Thakur C,Ellwart JW,Rapp UR,Ullrich A,Wagner E,Knyazev P,Roidl A

MA5-14488 was used in immunocytochemistry, immunohistochemistry, and western blot to study the killing of HER2-positive cancer cells and cancer stem cells by a combination of trastuzumab and salinomycin treatment of MCF-7 mammospheres

Sat Dec 15 00:00:00 EST 2012

The prognostic value of breast cancer resistance protein (BCRB/ABCG2) expression in breast carcinomas.

Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer

Omran OM

MA5-14488 was used in immunohistochemistry to study the prognostic utility of ABCG2 immunohistochemical expression in breast carcinomas

Tue Apr 02 00:00:00 EDT 2013

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