Invitrogen

ErbB2 (HER-2) Antibody Cocktail

Tech Support

Datasheet

Protocols

Tech Support

Antibody Testing Data (2)
Published Figures (1)

ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

ErbB2 (HER-2) Antibody (MA5-11976) in ICC/IF

Immunofluorescence analysis of HER-2/ErbB2 was done on 70% confluent log phase T47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HER-2/ErbB2 (L87 + 2ERB19) Mouse Monoclonal Antibody (Product # MA5-11976) at 2 µg/mL in 0.1% BSA and incubated for 3 h... View More

Product Image Gallery

ErbB2 (HER-2) Antibody in Western Blot (WB) — Western blot of HER-2 using HER-2 Monoclonal Antibody (Product # MA5-11976) on SKBR3 Cells.

ErbB2 (HER-2) Antibody in ChIP Assay (ChIP) — Published figure using ErbB2 (HER-2) cocktail antibody (Product # MA5-11976) in ChIP Assay

Product Details

MA5-11976

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Clone

L87, 2ERB19

Immunogen

Extracellular domain of recombinant human c-erbB-2 oncoprotein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10981681

Product Specific Information

MA5-11976 targets HER-2 in WB applications and shows reactivity with Human samples.

The MA5-11976 immunogen is extracellular domain of recombinant human c-erbB-2 oncoprotein.

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ganoderma tsugae Extract Inhibits Growth of HER2-Overexpressing Cancer Cells via Modulation of HER2/PI3K/Akt Signaling Pathway.

Evidence-based complementary and alternative medicine : eCAM

Kuo HP,Hsu SC,Ou CC,Li JW,Tseng HH,Chuang TC,Liu JY,Chen SJ,Su MH,Cheng YC,Chou WY,Kao MC

MA5-11976 was used in immunohistochemistry, immunoprecipitation, and western blot to study the role of the HER2-PI3 kinase-Akt pathway in the inhibitory effects of a Gandoderma tsugae extract on the growth of cancer cells overexpressing HER2

Mon May 13 00:00:00 EDT 2013

Defining the molecular response to trastuzumab, pertuzumab and combination therapy in ovarian cancer.

British journal of cancer

Sims AH,Zweemer AJ,Nagumo Y,Faratian D,Muir M,Dodds M,Um I,Kay C,Hasmann M,Harrison DJ,Langdon SP

MA5-11976 was used in western blot to study the molecular mechanisms underlying the therapeutic actions of trastuzumab and pertuzumab against ovarian cancer

Tue May 22 00:00:00 EDT 2012

Shed HER2 extracellular domain in HER2-mediated tumor growth and in trastuzumab susceptibility.

Journal of cellular physiology

Ghedini GC,Ciravolo V,Tortoreto M,Giuffrè S,Bianchi F,Campiglio M,Mortarino M,Figini M,Coliva A,Carcangiu ML,Zambetti M,Piazza T,Ferrini S,Ménard S,Tagliabue E,Pupa SM

MA5-11976 was used in western blot to study the role of the shed HER2 extracellular domain in HER2-mediated tumor growth and in trastuzumab susceptibility

Fri Oct 01 00:00:00 EDT 2010

Expression of estrogen receptor alpha with a Tet-off adenoviral system induces G0/G1 cell cycle arrest in SKBr3 breast cancer cells.

International journal of oncology

Peng J,Jordan VC

MA5-11976 was used in western blot to investigate the effect of estrogen receptor alpha on breast cancer proliferation and cell cycle progression

Mon Feb 01 00:00:00 EST 2010

Delivery of noncarrier naked DNA vaccine into the skin by supersonic flow induces a polarized T helper type 1 immune response to cancer.

The journal of gene medicine

Lin CC,Yen MC,Lin CM,Huang SS,Yang HJ,Chow NH,Lai MD

MA5-11976 was used in immunohistochemistry to investigate the immune response elicited by the skin delivery of naked DNA vaccine

Sun Jun 01 00:00:00 EDT 2008

Taxol increases the amount and T cell activating ability of self-immune stimulatory multimolecular complexes found in ovarian cancer cells.

Cancer research

Tsuda N,Chang DZ,Mine T,Efferson C,García-Sastre A,Wang X,Ferrone S,Ioannides CG

MA5-11976 was used in western blot to study the effect of taxol on tumor antigen-specific immunity in ovarian cancer cells

Sat Sep 01 00:00:00 EDT 2007

Curcumin-induced degradation of ErbB2: A role for the E3 ubiquitin ligase CHIP and the Michael reaction acceptor activity of curcumin.

Biochimica et biophysica acta

Jung Y,Xu W,Kim H,Ha N,Neckers L

MA5-11976 was used in western blot to study the molecular mechanism of curcumin-induced degradation of ErbB

Thu Mar 01 00:00:00 EST 2007

Biosynthesis of tumorigenic HER2 C-terminal fragments by alternative initiation of translation.

The EMBO journal

Anido J,Scaltriti M,Bech Serra JJ,Santiago Josefat B,Todo FR,Baselga J,Arribas J

MA5-11976 was used in western blot to study the generation of tumorigenic HER2 C-terminal fragments by alternative initiation of translation

Wed Jul 12 00:00:00 EDT 2006

Identification of ADAM10 as a major source of HER2 ectodomain sheddase activity in HER2 overexpressing breast cancer cells.

Cancer biology & therapy

Liu PC,Liu X,Li Y,Covington M,Wynn R,Huber R,Hillman M,Yang G,Ellis D,Marando C,Katiyar K,Bradley J,Abremski K,Stow M,Rupar M,Zhuo J,Li YL,Lin Q,Burns D,Xu M,Zhang C,Qian DQ,He C,Sharief V,Weng L,Agrios C,Shi E,Metcalf B,Newton R,Friedman S,Yao W,Scherle P,Hollis G,Burn TC

MA5-11976 was used in western blot to study the major role of ADAM10 in HER2 ectodomain shedding in HER2 overexpressing breast cancer cells

Thu Jun 01 00:00:00 EDT 2006

Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex.

Nature structural & molecular biology

Xu W,Yuan X,Xiang Z,Mimnaugh E,Marcu M,Neckers L

MA5-11976 was used in western blot to study the role of surface charge and hydrophobicity in ErbB2 binding to the Hsp90 chaperone complex

Tue Feb 01 00:00:00 EST 2005

Decreased accessibility and lack of activation of ErbB2 in JIMT-1, a herceptin-resistant, MUC4-expressing breast cancer cell line.

Cancer research

Nagy P,Friedländer E,Tanner M,Kapanen AI,Carraway KL,Isola J,Jovin TM

MA5-11976 was used in western blot to study the status of ErbB2 in a herceptin-resistant and MUC4-expressing breast cancer cell line

Sat Jan 15 00:00:00 EST 2005

Differential sensitivities of trastuzumab (Herceptin)-resistant human breast cancer cells to phosphoinositide-3 kinase (PI-3K) and epidermal growth factor receptor (EGFR) kinase inhibitors.

Breast cancer research and treatment

Chan CT,Metz MZ,Kane SE

MA5-11976 was used in immunoprecipitation to investigate the mechanism for the trastuzumab resistence in human breast cancer treatment

Sun May 01 00:00:00 EDT 2005

Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.

Cancer biotherapy & radiopharmaceuticals

Horak E,Heitner T,Robinson MK,Simmons HH,Garrison J,Russeva M,Furmanova P,Lou J,Zhou Y,Yuan QA,Weiner LM,Adams GP,Marks JD

MA5-11976 was used in western blot to develop and characterize specific antibodies targeting EGFR family

Thu Dec 01 00:00:00 EST 2005

Alpha6beta1 integrin induces proteasome-mediated cleavage of erbB2 in breast cancer cells.

Oncogene

Shimizu H,Seiki T,Asada M,Yoshimatsu K,Koyama N

MA5-11976 was used in western blot to study the role of alpha6beta1 integrin in inducing proteasome-mediated cleavage of erbB2 in breast cancer cells

Thu Feb 13 00:00:00 EST 2003

Role of MHC II affinity and molecular mimicry in defining anti-HER-2/neu MAb-3 linear peptide epitope.

Peptides

Lucchese A,Stevanovic S,Sinha AA,Mittelman A,Kanduc D

MA5-11976 was used in western blot to study whether it is possible to predict which peptides will evoke a humoral immune response using the human HER-2/neu breast cancer-associated antigen as a model

Sat Feb 01 00:00:00 EST 2003

Chaperone-dependent E3 ubiquitin ligase CHIP mediates a degradative pathway for c-ErbB2/Neu.

Proceedings of the National Academy of Sciences of the United States of America

Xu W,Marcu M,Yuan X,Mimnaugh E,Patterson C,Neckers L

MA5-11976 was used in western blot to investigate the influence of CHIP on c-ErbB2/Neu degradation

Tue Oct 01 00:00:00 EDT 2002

Chaperone-dependent E3 ubiquitin ligase CHIP mediates a degradative pathway for c-ErbB2/Neu.

Proceedings of the National Academy of Sciences of the United States of America

Xu W,Marcu M,Yuan X,Mimnaugh E,Patterson C,Neckers L

MA5-11976 was used in western blot to investigate the influence of CHIP on c-ErbB2/Neu degradation

Tue Oct 01 00:00:00 EDT 2002

Monoclonal and polyclonal humoral immune response to EC HER-2/NEU peptides with low similarity to the host's proteome.

International journal of cancer

Mittelman A,Lucchese A,Sinha AA,Kanduc D

MA5-11976 was used in western blot to study the immune response to EC HER-2/NEU peptides not shared by the host's proteome

Wed Apr 10 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: c-erb B2/neu protein; CD antigen CD340; CD340; erb-b2; erbb 2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; herstatin; human epidermal growth factor receptor 2; metas; Metastatic lymph node gene 19 protein; MLN 19; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; phospho ErbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

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Gene Aliases: CD340; ERBB2; HER-2; HER-2/neu; HER2; MLN 19; MLN19; NEU; NGL; TKR1

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UniProt ID: (Human) P04626

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Entrez Gene ID: (Human) 2064

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Molecular Function: transmembrane signal receptor

Function(s)

Process(es)

RNA polymerase I core binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding protein C-terminus binding growth factor binding protein phosphatase binding identical protein binding ErbB-3 class receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity protein dimerization activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ErbB2 (HER-2) Antibody Cocktail

ErbB2 (HER-2) Antibody (MA5-11976) in ICC/IF

Product Details

MA5-11976

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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