Invitrogen

ErbB4 Polyclonal Antibody

Product Image Gallery

ErbB4 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ERBB4 showing staining in the cytoplasm and nucleus of MCF-7 cells. MCF-7 cells were fixed in ice-cold MeOH for 5 min and stained using an ERBB4 polyclonal antibody (Product # PA5-29272) diluted at 1:500. Blue: Hoechst 33342 staining.

ErbB4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ERBB4 in paraffin-embedded NCI-N87 xenograft using an ERBB4 polyclonal antibody (Product # PA5-29272) at a dilution of 1:500.

ErbB4 Antibody in Western Blot (WB) — Western blot analysis of ERBB4 using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an ERBB4 polyclonal antibody (Product # PA5-29272) at a dilution of 1:1000.

ErbB4 Antibody in Immunoprecipitation (IP) — PA5-29272 immunoprecipitates ERBB4 protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 4 µg of preimmune Rabbit IgG B. Immunoprecipitation of ERBB4 protein by 4 µg ERBB4 antibody (Product # PA5-29272) on 5% SDS-PAGE.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

Assay-Dependent

Western Blot (WB)

1:1000-1:10,000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 850 and 1101 of Human ERBB4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.78 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546748

Product Specific Information

PA5-29272 targets ERBB4 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.

The PA5-29272 immunogen is recombinant fragment corresponding to a region within amino acids 850 and 1101 of Human ERBB4.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

ErbB 4 (Her-4, c-erbB-4, v-erbB-4) is a member of the class I receptor kinase family. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 4ICD; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; ERBB4 intracellular domain; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; HER4; human epidermal growth factor receptor 4; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; s80HER4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

Gene Aliases: ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4

UniProt ID: (Human) Q15303, (Mouse) Q61527

Entrez Gene ID: (Human) 2066, (Mouse) 13869

Function(s)