Invitrogen

ErbB4 Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (6)

ErbB4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

ErbB4 Antibody (PA5-29272) in ICC/IF

ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: ERBB4 protein stained by ERBB4 antibody [C1C3] (Product # PA5-29272) diluted at 1:500. Blue: Hoechst 33342 staining.

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ErbB4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ERBB4 (v-erb-A) (Product # PA5-29272) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

ErbB4 Antibody in Western Blot (WB) — Western Blot analysis of ErbB4 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ErbB4 Polyclonal Antibody (Product # PA5-29272) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ErbB4 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Her4 / ErbB4 protein from MCF-7 whole cell extract using 4.5 µg of ErbB4 Polyclonal Antibody (Product # PA5-29272). Western blot analysis was performed using ErbB4 Polyclonal Antibody (Product # PA5-29272) and diluted at 1:500. HRP-conjugated anti rabbit IgG antibody was used to detect the primary antibody.

Product Details

PA5-29272

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 850 and 1101 of Human ERBB4

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.97 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546748

Product Specific Information

Recommended positive controls: A431, mouse brain, rat brain.

Predicted reactivity: Mouse (89%), Rat (97%), Xenopus laevis (86%), Chicken (88%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 4ICD; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; c-erbB-4; ERBB4 intracellular domain; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; Receptor tyrosine-protein kinase erbB-4; s80HER4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

Gene Aliases: ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4; Tyro-2

UniProt ID: (Human) Q15303, (Mouse) Q61527, (Rat) Q62956

Entrez Gene ID: (Human) 2066, (Mouse) 13869, (Rat) 59323

Molecular Function: transmembrane signal receptor