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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Bovine, Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Estrogen receptors from calf uterus.|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Endometrial expression of progesterone, estrogen, and oxytocin receptors and of 20¿-hydroxysteroid dehydrogenase and cyclooxygenase II 2 and 5 days after ovulation in induced short and normal estrous cycles in dairy cows.
MA1-12600 was used in immunohistochemistry to study potential mechanisms to explain the short estrous cycles often observed in dairy cows following synchronization of estrus
|Rantala MH,Mutikainen M,Schuler G,Katila T,Taponen J||Theriogenology (81:1181)||2014|
A comparison of oestrogen receptor and progesterone receptor expression in endometrial polyps and endometrium of premenopausal women.
MA1-12600 was used in immunohistochemistry to study the expression of sex hormone receptors in endometrial polyps and surrounding endometrial tissue
|Peng X,Li T,Xia E,Xia C,Liu Y,Yu D||Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology (29:340)||2009|
Estrogen receptor alpha and beta immunoreactive neurons in normal adult and aged female rat hippocampus: a qualitative and quantitative study.
MA1-12600 was used in immunohistochemistry to investigate the expression of estrogen in the rat hippocampus
|Mehra RD,Sharma K,Nyakas C,Vij U||Brain research (1056:22)||2005|
Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.
MA1-12600 was used in immunohistochemistry to study the immunohistochemical expression of estrogen receptor-alpha in bovine placentomes
|Schuler G,Wirth C,Teichmann U,Failing K,Leiser R,Thole H,Hoffmann B||Biology of reproduction (66:976)||2002|
Expression of estrogen receptor variant messenger RNAs and determination of estrogen receptor status in human breast cancer.
MA1-12600 was used in immunohistochemistry to investigate the mRNA isoform expression of estrogen receptor and its effect on the determination of estrogen receptor level in human breast cancer
|Huang A,Leygue ER,Snell L,Murphy LC,Watson PH||The American journal of pathology (150:1827)||1997|
Guggulsterone-mediated enhancement of radiosensitivity in human tumor cell lines.
MA1-12600 was used in western blot to study the effects of guggulsterone on the radiosensitivity of human tumor cell lines
|Choudhuri R,Degraff W,Gamson J,Mitchell JB,Cook JA||Frontiers in oncology (1:null)||2012|
Estrogen-dependent signaling in a molecularly distinct subclass of aggressive prostate cancer.
MA1-12600 was used in western blot to investigate the molecular signature of TMPRSS2-ERG gene fusions in prostate cancers
|Setlur SR,Mertz KD,Hoshida Y,Demichelis F,Lupien M,Perner S,Sboner A,Pawitan Y,Andrén O,Johnson LA,Tang J,Adami HO,Calza S,Chinnaiyan AM,Rhodes D,Tomlins S,Fall K,Mucci LA,Kantoff PW,Stampfer MJ,Andersson SO,Varenhorst E,Johansson JE,Brown M,Golub TR,Rubin MA||Journal of the National Cancer Institute (100:815)||2008|
HER-2/neu x aromatase double transgenic mice model: the effects of aromatase overexpression on mammary tumorigenesis.
MA1-12600 was used in western blot to study the importance of local estrogen in oncogene-mediated breast tumorigenesis using a HER-2/neu x aromatase double transgenic mice model
|Tekmal RR,Nair HB,Perla RP,Kirma N||The Journal of steroid biochemistry and molecular biology (106:111)||2007|
The tumor suppressor activity of the lysyl oxidase propeptide reverses the invasive phenotype of Her-2/neu-driven breast cancer.
MA1-12600 was used in western blot to investigate the influence of lysyl oxidase propeptid on breast tumor invasiveness
|Min C,Kirsch KH,Zhao Y,Jeay S,Palamakumbura AH,Trackman PC,Sonenshein GE||Cancer research (67:1105)||2007|
Tuberin nuclear localization can be regulated by phosphorylation of its carboxyl terminus.
MA1-12600 was used in western blot to study the regulation of tuberin nuclear localization by phosphorylation of its carboxyl terminus
|York B,Lou D,Noonan DJ||Molecular cancer research : MCR (4:885)||2006|
Hyaluronan-CD44 interaction with IQGAP1 promotes Cdc42 and ERK signaling, leading to actin binding, Elk-1/estrogen receptor transcriptional activation, and ovarian cancer progression.
MA1-12600 was used in western blot to investigate the effect of the interaction between hyaluronan-CD44 and IQGAP1 on cdc42and ERK signaling
|Bourguignon LY,Gilad E,Rothman K,Peyrollier K||The Journal of biological chemistry (280:11961)||2005|
Conjugated linoleic acid blocks estrogen signaling in human breast cancer cells.
MA1-12600 was used in western blot to investigate the mechanism for the anti-tumor activity of conjugated linoleic acid
|Tanmahasamut P,Liu J,Hendry LB,Sidell N||The Journal of nutrition (134:674)||2004|
Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens.
MA1-12600 was used in immunocytochemistry to validate a method for the measurement of HER2 total protein and HER2 homodimers in FFPE breast cancer tumor specimens
|Larson JS,Goodman LJ,Tan Y,Defazio-Eli L,Paquet AC,Cook JW,Rivera A,Frankson K,Bose J,Chen L,Cheung J,Shi Y,Irwin S,Kiss LD,Huang W,Utter S,Sherwood T,Bates M,Weidler J,Parry G,Winslow J,Petropoulos CJ,Whitcomb JM||Pathology research international (2010:null)||2010|
Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography.
MA1-12600 was used in EMSA to develop a method for the affinity purification of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae
|Feng W,Graumann K,Hahn R,Jungbauer A||Journal of chromatography. A (852:161)||1999|
ER; ER-alpha; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; Nuclear receptor subfamily 3 group A member 1
ER; ER-alpha; ERa; ERalpha; ESR; ESR1; ESRA; Estr; Estra; ESTRR; NR3A1