Western blot analysis of Estrogen-Related Receptor gamma was performed by loading 25 ug of MCF-7 (Lane 1), 293T (Lane 2), and Mouse kidney (Lane 3) cell lysates and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4°C overnight. The membrane was probed with an Estrogen-Related Receptor gamma polyclonal antibody (Product # PA1-316) at a dilution of 1:500 (MCF-7 and 293T) and 1:1000 (Mouse kidney) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Results show a band at 54 kDa in MCF-7 and Mouse kidney cell lysates.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D(401) Y E A G Q H M E D P R R(413) of human ERR3.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-316 detects human and mouse ERR3 from transfected cells and shows no cross-reactivity to ERR1.
PA1-316 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~54 kDa protein representing ERR1 from COS-7 transfected cells. This antibody is not suitable for 293T cells in Western blot analysis.
PA1-316 immunogen is a synthetic peptide corresponding to residues D(401) Y E A G Q H M E D P R R(413) of human ERR3. This sequence is completely conserved between human, mouse and rat. This immunizing peptide (PEP-232) can be used for neutralization and control experiments.
Estrogen-related receptor gamma (ERR gamma, ERR3, NR3B3) is an orphan member of the nuclear hormone receptor superfamily. All the ERR family members (1,2, and 3) share an almost identical DNA-binding domain, which has 68% amino acid identity with that of estrogen receptor. Studies involving the crystal structure of the LBD have shown a complex between ERR3 and SRC-1. ERR3 binds as a homodimer to direct repeats (5 and quote;-AGG TCA-3 and quote;), to extended half-sites, and to the inverted estrogen response element.
The corresponding gene for the ERR3 is NR3B3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Estrogen-related receptor ¿ (ERR¿) regulates oxygen-dependent expression of voltage-gated potassium (K+) channels and tissue kallikrein during human trophoblast differentiation.
PA1-316 was used in ChIP assay to study the expression of tissue kallikrein and KV7 channels and the regulatory role of estrogen-related receptor gamma and oxygen tension during trophoblast differentiation
|Luo Y,Kumar P,Mendelson CR||Molecular endocrinology (Baltimore, Md.) (27:940)||2013|
Estrogen-related receptors-stimulated monoamine oxidase B promoter activity is down-regulated by estrogen receptors.
PA1-316 was used in chromatin immunoprecipitation to study the effect of estrogen receptors on ERR-stimulated monoamine oxidase B promoter activity.
|Zhang Z,Chen K,Shih JC,Teng CT||Molecular endocrinology (Baltimore, Md.) (20:1547)||2006|
Estrogen-related receptor gamma (ERRgamma) mediates oxygen-dependent induction of aromatase (CYP19) gene expression during human trophoblast differentiation.
PA1-316 was used in western blot to investigate the role of estrogen-related receptor gamma on aromatase (CYP19) gene expression during human trophoblast differentiation
|Kumar P,Mendelson CR||Molecular endocrinology (Baltimore, Md.) (25:1513)||2011|
Identification and characterization of estrogen receptor-related receptor alpha and gamma in human glioma and astrocytoma cells.
PA1-316 was used in western blot to characterize estrogen receptor-related receptor alpha and gamma in glioma and astrocytoma cells
|Gandhari MK,Frazier CR,Hartenstein JS,Cloix JF,Bernier M,Wainer IW||Molecular and cellular endocrinology (315:314)||2010|