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Immunohistochemistry was performed on formalin -fixed paraffin-embedded rat infarct heart tissue sections. To expose target proteins, heat-induced epitope retrieval (HIER) was performed in sodium citrate buffer (pH 6.0) for 30 minutes at 100°C. Tissues were blocked in 10% normal goat serum for 20 minutes at room temperature and probed with a F4/80 monoclonal antibody (Product # MA5-16363) at a dilution of 1:100 for 1 hour at room temperature. Tissues were washed extensively with 1X PBS. Detection was performed using a biotinylated goat anti-rabbit IgG secondary antibody followed by an avidin-biotin complex reagent and DAB colorimetric substrate. Tissues were counterstained with hematoxilyn and visualized by light microscopy. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from N-terminus of mouse F4/80 protein.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is human lung or mouse liver tissue.
Mouse EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) or cell surface glycoprotein F4/80 is a cell surface glycoprotein and a member of the EGF TM7 family of proteins. It shares 68% overall amino acid identity with human EGF-module-containing-mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ regulatory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cell surface glycoprotein F4/80; DD7A5-7; egf-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
ADGRE1; DD7A5-7; EGF-TM7; EMR1; F4/80; Gpf480; Ly71; TM7LN3