Immunohistochemistry analysis of FGFR1 / CD331 showing staining in the cytoplasm and membrane of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a FGFR1 / CD331 monoclonal antibody (133100) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Bovine, Chicken, Guinea pig, Human, Mouse, Rat|
|Published species reactivity||Rabbit, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bovine FGF Receptor 1 (flg) purified from bovine Coronary Venular Endothelial Cells (CVEC).|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Immunoprecipitation (IP)||2-5 ug|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
FGFR1 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
L1CAM stimulates glioma cell motility and proliferation through the fibroblast growth factor receptor.
13-3100 was used in western blot to elucidate the relationship between LCAM cell adhesion/recognition molecule and fibroblast growth factor receptor in high-grade glioma cells.
|Mohanan V,Temburni MK,Kappes JC,Galileo DS||Clinical and experimental metastasis (30:507)||2013|
|Human||Not Cited||Fragile histidine triad expression delays tumor development and induces apoptosis in human pancreatic cancer.||Dumon KR,Ishii H,Vecchione A,Trapasso F,Baldassarre G,Chakrani F,Druck T,Rosato EF,Williams NN,Baffa R,During MJ,Huebner K,Croce CM||Cancer research (61:4827)||2001|
|Rabbit||Not Cited||Fibroblast growth factor and epidermal growth factor receptors in ligament healing.||Panossian V,Liu SH,Lane JM,Finerman GA||Clinical orthopaedics and related research (null:173)||1997|