Immunofluorescent analysis of FGFR4/CD334 in HeLa cells using a FGFR4/CD334 polyclonal antibody (Product # PA1-31991). Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min) and incubated with primary antibody ata dilution of 1:200 for 2 hrs at room temperature. Detection was performed using an Alexa Fluor 488 conjugated donkey anti-rabbit secondary antibody (green) at a dilution of 1:1000 for 1 hr. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH, corresponding to amino acids 39-56 of Human FGFR 4.|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31991 detects FGFR4 from human samples.
PA1-31991 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot and ELISA applications. Detects a band of ~96 kDa. A suggested positive control for this product is THP-1 cell lysate.
The PA1-31991 immunogen is a synthetic peptide conjugated to KLH, corresponding to amino acids 39-56 of Human FGFR 4.
Store at 4°C for short term. For extended storage aliquot and store at -20°C or below. Avoid Freeze/Thaw Cycles.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.