Invitrogen

FIG4 Polyclonal Antibody

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FIG4 Antibody in Western Blot (WB) — Western Blot analysis of FIG4 in various cell lines using a FIG4 Polyclonal antibody (Product # PA5-120353) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.

Product Details

PA5-120353

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200 - 1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 608-907 of human FIG4 (NP_055660.1). View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.72 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2913925

Product Specific Information

Positive test controls include: U-251MG, HT-29, Jukat, HeLa, Mouse liver, Mouse spleen. The target is usually found in the following locations: Endosome membrane.

Target Information

The protein encoded by this gene belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease, type 4J.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FIG4 homolog, SAC domain containing lipid phosphatase; FIG4 homolog, SAC1 domain containing lipid phosphatase; FIG4 homolog, SAC1 lipid phosphatase domain containing; Phosphatidylinositol 3,5-bisphosphate 5-phosphatase; Polyphosphoinositide phosphatase; RP1-249I4.1; Sac domain-containing inositol phosphatase 3; SAC domain-containing protein 3

Gene Aliases: A530089I17Rik; AI326867; ALS11; BTOP; CMT4J; dJ249I4.1; FIG4; KIAA0274; SAC3; YVS

UniProt ID: (Human) Q92562, (Mouse) Q91WF7

Entrez Gene ID: (Human) 9896, (Mouse) 103199

Molecular Function: phosphatase