Invitrogen

FKBP5 Polyclonal Antibody

Product Image Gallery

FKBP5 Antibody — Figure 6. WD increases FKBP51 protein levels in the hippocampus. A , Representative Western blot shows increased FKBP51 levels in the brains of the rats that consumed the WD. B , Analyses showed a significant main effect of the diet on brain FKBP51 levels (diet effect: F (1,19) = 15.45, p = 0.0009), PS exposure ( F (1,19) = 0.51, p = 0.49), or interaction between diet and PS exposure ( F (1,19) = 0.42, p = 0.52) did not show significant main effects on FKBP51 levels. C , Representative blot from micropunched anxiety-related brain regions showed distinctive FKBP51 levels. D , Analyses revealed a significant increase in FKBP51 levels in the hippocampus of the WDE rats when compared with CDE rats ( F (1,16) = 9.88; p = 0.0063, p = 0.021). Diet did not alter FKBP51 levels in the amygdala or medial prefrontal cortex. Although it did not reach statistical significance, we found a trend for more prefrontal cortex FKBP51 levels when compared with the amygdala and the hippocampus ( F (2,16) = 3.58, p = 0.052). No significant interactions between diet and the studied brain regions were revealed ( F (2,16) = 2.44, p = 0.12). Data are expressed as the mean +- SEM. * p < 0.05. Amygdala (AMY), n = 3-4 rats; medial prefrontal cortex (mPFC), n = 3-4; HPC, n = 4-5.

FKBP5 Antibody in Western Blot (WB) — Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of K562 (Lane 1), Hep G2 (Lane 2), PC3 (Lane 3), and MCF-7 (Lane 4). The blots were probed with Anti-FKBP51 Rabbit Polyclonal Antibody (Product # PA1-020, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Approximately 51 and 45 kDa bands corresponding to FKBP51 were observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

Tested Applications

Dilution

Western Blot (WB)

1-2 µg/mL

Published Applications

Western Blot (WB)

See 13 publications below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the residues C(394) Q K K A K E H N E R D R R(407) of human FKBP51.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2103140

Product Specific Information

PA1-020 detects FK506 binding protein 51 kDa (FKBP51) in canine, hamster, human, mouse and rat tissues.

PA1-020 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~51 kDa protein representing FKBP51 in HeLa cell lysate. By Western blot PA1-020 also detects FKBP51 in whole tissue extracts from rat kidney and rat and mouse testes.

The PA1-020 immunogen is a synthetic peptide corresponding to the residues C(394) Q K K A K E H N E R D R R(407) of human FKBP51. There is a single amino acid substitution (K396 to R396) in this domain between human and mouse FKBP51 protein. PA1-020 immunizing peptide (Cat. # PEP-078) is available for use in neutralization and control experiments.

Target Information

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.

FK506 binding protein 51 kDa (FKBP51) is found associated with the progesterone receptor. This protein is also referred to as FKBP54 in the literature. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 51 kDa FK506-binding protein; 51 kDa FKBP; 54 kDa progesterone recepto; 54 kDa progesterone receptor-associated immunophilin; Androgen-regulated protein 6; EC 5.2.1.8; FF1 antigen; FK506 binding protein 5; FK506 Binding Protein 51; FK506-binding protein 5; FKBP-5; FKBP-51; FKBP54; HSP90-binding immunophilin; p54; Peptidyl-prolyl cis-trans isomerase FKBP5; peptidylprolyl cis-trans isomerase; PPIase FKBP5; Rotamase; T-cell FK506-binding protein

Gene Aliases: AIG6; D17Ertd592e; Dit1; FKBP-5; FKBP5; FKBP51; FKBP54; P54; PPIase; Ptg-10

UniProt ID: (Human) Q13451, (Mouse) Q64378

Entrez Gene ID: (Human) 2289, (Dog) 481759, (Mouse) 14229, (Rat) 361810, (Chinese hamster) 100760904

Molecular Function: calcium-binding protein chaperone isomerase