Invitrogen

FKBP5 Polyclonal Antibody

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FKBP5 Antibody in Western Blot (WB) — Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of K562 (Lane 1), Hep G2 (Lane 2), PC3 (Lane 3), and MCF-7 (Lane 4). The blots were probed with Anti-FKBP51 Rabbit Polyclonal Antibody (Product # PA1-020, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Approximately 51 and 45 kDa bands corresponding to FKBP51 were observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

FKBP5 Antibody in Immunohistochemistry (IHC) — Published figure using FKBP5 polyclonal antibody (Product # PA1-020) in Immunohistochemistry

Product Details

PA1-020

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 17 publications 17 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Hamster, Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to the residues C(394) Q K K A K E H N E R D R R(407) of human FKBP51. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2103140

Product Specific Information

PA1-020 detects FK506 binding protein 51 kDa (FKBP51) in canine, hamster, human, mouse and rat tissues.

PA1-020 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~51 kDa protein representing FKBP51 in HeLa cell lysate. By Western blot PA1-020 also detects FKBP51 in whole tissue extracts from rat kidney and rat and mouse testes.

The PA1-020 immunogen is a synthetic peptide corresponding to the residues C(394) Q K K A K E H N E R D R R(407) of human FKBP51. There is a single amino acid substitution (K396 to R396) in this domain between human and mouse FKBP51 protein. PA1-020 immunizing peptide (Cat. # PEP-078) is available for use in neutralization and control experiments.

Target Information

FKBP5 is a member of the immunophilin protein family. This family plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP5 is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. The gene that encodes FKBP5 has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lu Y,Sareddy GR,Wang J,Zhang Q,Tang FL,Pratap UP,Tekmal RR,Vadlamudi RK,Brann DW

PA1-020 was used in Western Blotting to demonstrate neuron-derived 17β-estradiol has a neuroprotective role during ischemic brain injury.

Wed Sep 16 00:00:00 EDT 2020

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lu Y,Sareddy GR,Wang J,Zhang Q,Tang FL,Pratap UP,Tekmal RR,Vadlamudi RK,Brann DW

PA1-020 was used in Western Blotting to demonstrate neuron-derived 17β-estradiol has a neuroprotective role during ischemic brain injury.

Wed Sep 16 00:00:00 EDT 2020

Nucleocytoplasmic shuttling of the glucocorticoid receptor is influenced by tetratricopeptide repeat-containing proteins.

Journal of cell science

Mazaira GI,Echeverria PC,Galigniana MD

PA1-020 was used in Western Blotting to show that nuclear import of GR was impaired, whereas GR nuclear export was enhanced.

Tue Jun 16 00:00:00 EDT 2020

Heme Oxygenase 1 Impairs Glucocorticoid Receptor Activity in Prostate Cancer.

International journal of molecular sciences

Leonardi DB,Anselmino N,Brandani JN,Jaworski FM,Páez AV,Mazaira G,Meiss RP,Nuñez M,Nemirovsky SI,Giudice J,Galigniana M,Pecci A,Gueron G,Vazquez E,Cotignola J

PA1-020 was used in Western Blotting to identify the molecular processes governed by the interaction between haem oxygenase 1 and glucocorticoid receptor.

Tue Feb 26 00:00:00 EST 2019

In silico identification and biochemical characterization of the human dicarboxylate clamp TPR protein interaction network.

FEBS open bio

Bernadotte A,Kumar R,Winblad B,Pavlov PF

PA1-020 was used in Western Blot to identify and characterise the human dicarboxylate clamp tetratricopeptide repeat protein interaction network.

Thu Nov 01 00:00:00 EDT 2018

Western High-Fat Diet Consumption during Adolescence Increases Susceptibility to Traumatic Stress while Selectively Disrupting Hippocampal and Ventricular Volumes.

eNeuro

Kalyan-Masih P,Vega-Torres JD,Miles C,Haddad E,Rainsbury S,Baghchechi M,Obenaus A,Figueroa JD

PA1020 was used in western blot to test if a Western-like high-fat diet predisposes rats to post-traumatic stress responsivity

Thu Oct 26 00:00:00 EDT 2017

Pharmacological stimulation of hypoxia inducible factor-1α facilitates the corticosterone response to a mild acute stressor.

Neuroscience letters

Harrell CS,Rowson SA,Neigh GN

PA1-020 was used in western blot to determine if HIF-1 links glucocorticoids and oxidative stress

Thu Jul 23 00:00:00 EDT 2015

Subcellular rearrangement of hsp90-binding immunophilins accompanies neuronal differentiation and neurite outgrowth.

Journal of neurochemistry

Quintá HR,Maschi D,Gomez-Sanchez C,Piwien-Pilipuk G,Galigniana MD

PA1-020 was used in immunocytochemistry and western blot to investigate the alterations hsp90-binding immunophilins distribution during neuronal growth and differentiation

Mon Nov 01 00:00:00 EDT 2010

Subcellular rearrangement of hsp90-binding immunophilins accompanies neuronal differentiation and neurite outgrowth.

Journal of neurochemistry

Quintá HR,Maschi D,Gomez-Sanchez C,Piwien-Pilipuk G,Galigniana MD

PA1-020 was used in immunocytochemistry and western blot to investigate the alterations hsp90-binding immunophilins distribution during neuronal growth and differentiation

Mon Nov 01 00:00:00 EDT 2010

PHLPP1 splice variants differentially regulate AKT and PKCα signaling in hippocampal neurons: characterization of PHLPP proteins in the adult hippocampus.

Journal of neurochemistry

Jackson TC,Verrier JD,Semple-Rowland S,Kumar A,Foster TC

PA1-020 was used in western blot to investigate the functions of different PHLPP splice variants in adult hippocampus

Mon Nov 01 00:00:00 EDT 2010

The hsp90-FKBP52 complex links the mineralocorticoid receptor to motor proteins and persists bound to the receptor in early nuclear events.

Molecular and cellular biology

Galigniana MD,Erlejman AG,Monte M,Gomez-Sanchez C,Piwien-Pilipuk G

PA1-020 was used in western blot to investigate the role of the heat shock protein 90-FKBP52 complex in the nucleus

Mon Mar 01 00:00:00 EST 2010

Comparative analysis of calcineurin inhibition by complexes of immunosuppressive drugs with human FK506 binding proteins.

Biochemistry

Weiwad M,Edlich F,Kilka S,Erdmann F,Jarczowski F,Dorn M,Moutty MC,Fischer G

PA1-020 was used in western blot to study the inhibition of calcineurin by immunosuppressive drug-FK506 binding protein complexes

Tue Dec 26 00:00:00 EST 2006

Up-regulation of glucocorticoid-regulated genes in a mouse model of Rett syndrome.

Human molecular genetics

Nuber UA,Kriaucionis S,Roloff TC,Guy J,Selfridge J,Steinhoff C,Schulz R,Lipkowitz B,Ropers HH,Holmes MC,Bird A

PA1-020 was used in western blot to study the expression of glucocorticoid-regulated genes in Rett syndrome mouse model.

Mon Aug 01 00:00:00 EDT 2005

Ethanol-responsive brain region expression networks: implications for behavioral responses to acute ethanol in DBA/2J versus C57BL/6J mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kerns RT,Ravindranathan A,Hassan S,Cage MP,York T,Sikela JM,Williams RW,Miles MF

PA1-020 was used in western blot to investigate the gene expression profile in brain regions of the mesolimbic reward pathway of mouse strains DBA/2J and C57BL/6J .

Wed Mar 02 00:00:00 EST 2005

Differential control of glucocorticoid receptor hormone-binding function by tetratricopeptide repeat (TPR) proteins and the immunosuppressive ligand FK506.

Biochemistry

Davies TH,Ning YM,Sánchez ER

PA1-020 was used in western blot to investigate the regulatory effects of FK506 and TPR proteins on glucocorticoid receptor hormone-binding function

Tue Feb 15 00:00:00 EST 2005

Association of immunophilins with mammalian TRPC channels.

The Journal of biological chemistry

Sinkins WG,Goel M,Estacion M,Schilling WP

PA1-020 was used in western blot to study the role of immunophilins in TRPC channel activation following receptor stimulation.

Fri Aug 13 00:00:00 EDT 2004

Progesterone receptor deficient in chromatin binding has an altered cellular state.

The Journal of biological chemistry

Botos J,Xian W,Smith DF,Smith CL

PA1-020 was used in western blot to analyze the altered cellular processing by comparing the composition and distribution of tPR and sPR complexes

Fri Apr 09 00:00:00 EDT 2004

A new first step in activation of steroid receptors: hormone-induced switching of FKBP51 and FKBP52 immunophilins.

The Journal of biological chemistry

Davies TH,Ning YM,Sánchez ER

PA1-020 was used in western blot to investigate the activation mechanism for glucocorticoid receptors.

Fri Feb 15 00:00:00 EST 2002

Tissue distribution and abundance of human FKBP51, and FK506-binding protein that can mediate calcineurin inhibition.

Biochemical and biophysical research communications

Baughman G,Wiederrecht GJ,Chang F,Martin MM,Bourgeois S

PA1-020 was used in western blot to study the expression of FK506-binding protein FKBP51 in tissues

Mon Mar 17 00:00:00 EST 1997

Bioinformatics

Protein Aliases: 51 kDa FK506-binding protein; 51 kDa FKBP; 54 kDa progesterone receptor-associated immunophilin; Androgen-regulated protein 6; EC 5.2.1.8; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; FKBP-5; FKBP-51; FKBP54; HSP90-binding immunophilin; p54; Peptidyl-prolyl cis-trans isomerase FKBP5; peptidylprolyl cis-trans isomerase; PPIase FKBP5; Rotamase; T-cell FK506-binding protein

Gene Aliases: AIG6; D17Ertd592e; Dit1; FKBP-5; FKBP5; FKBP51; FKBP54; P54; PPIase; Ptg-10

UniProt ID: (Human) Q13451, (Mouse) Q64378

Entrez Gene ID: (Human) 2289, (Dog) 481759, (Mouse) 14229, (Rat) 361810, (Chinese hamster) 100760904

Molecular Function: chaperone