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Western blot analysis of FOXP3D and full-length (wild-type) transfected 293 cell lysate using a FOXP3D2 (exon 2 deleted) monoclonal antibody (Product # MA5-16226) and a FOXP3/Scurfin monoclonal antibody (Product # MA5-16222). At 1 ug/ml, a FOXP3D2 (exon 2 deleted) monoclonal antibody (Product # MA5-16226) specifically detects the FOXP3 (Lane 1); it does not detect full-length FOXP3 (Lane 2). In contrast, a FOXP3/Scurfin monoclonal antibody (Product # MA5-16222) detects both FOXP3D (Lane 3) and full-length (Lane 4) FOXP3.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||synthetic peptide corresponding to the exon 2 deletion site was used to immunize mice.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
One of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells or Tregs. Several elegant experiments using transgenic mice and retrovirus-mediated over expression studies, have led to the identification of FoxP3, a transcription factor, as a specific molecular marker essential for the development and function of Tregs. The primary evidence regarding the involvement of FoxP3 in the development of Tregs was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of Tregs and the role of FoxP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AIID; DIETER; enteropathy; Forkhead box protein P3; FOXP3delta7; immune dysregulation; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; PIDX; polyendocrinopathy; Scurfin; X-linked; XPID
AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID