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Western blot analysis of ferredoxin reductase was performed by loading 20ug of whole cell lysates from asynchronous (lane 1) and quiescent (lane 2) A549 cells, and 15ug of plasma membrane fractions from asynchronous (lane 3) and quiescent (lane 4) A549 cells per well onto a 10% SDS-PAGE gel. Cells were made quiescent by culturing in the absence of serum for 48 hours. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA in TBST for 1 hour. The membrane was probed with a Ferredoxin Reductase monoclonal antibody (Product # LF-MA0033) at a dilution of 1:500 in 2% BSA in TBST for 1 hour at room temperature, washed in TBST, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:2500 for 1 hour. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, recombinant, human ferredoxin reductase protein expressed in E. coli.|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HeLa, Jurkat or 293T.
Ferredoxin reductase is a ubiquitous flavoenzyme, containing noncovalently bound FAD as a prosthetic group. It plays a role in delivering NADPH or low potential one-electron donors such as ferredoxin and flavodoxin to redox-based metabolisms in plastids, mitochondria and bacteria. In mammals, ferredoxin reductase is loosely associated with the inner mitochondrial membrane and receives electrons from NADPH. These electrons are transferred to ferredoxin which shuttles electrons to cytochrome P450 in the adrenal cortex mitochondrial steroid hydroxylation systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
adrenodoxin reductase; adrenodoxin-NADP(+) reductase; ADXR; AR; ferredoxin--NADP(+) reductase