Invitrogen

Filamin A Monoclonal Antibody (FLMN01 (PM6/317))

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5 Published Figures

4 References

View all (33) Filamin A antibodies

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FIGURE: 1 / 13

Filamin A Antibody (MA5-11705) in WB

Western blot was performed using Anti-Filamin A Monoclonal Antibody (FLMN01 (PM6/317)) (Product # MA5-11705) and a 180kDa band corresponding to Filamin A was observed across all cell lines tested except LNCaP and HEK-293 which are known to have low expression of Filamin A (DOI: 10.18632/oncotarget.9472). Whole cell extracts (50 µg lysate) of DU 145 (Lane 1), LNCaP (Lane 2), HeLa (Lane 3), HEK-293 (Lane 4) and A549 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then trans... View More

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Filamin A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Filamin A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Filamin A Monoclonal Antibody (FLMN01 (PM6/317)) (Product # MA5-11705) at 2µg/mL dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly cytoplasmic localization. Panel e represents HEK-293 cells showing lower expression of Filamin A. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Filamin A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human skin stained with Filamin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscle cells surrounding blood vessels.

Filamin A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Filamin A was observed in DU 145, HeLa and A549 as compared to LNCaP and HEK-293 which are known to have lower expression of Filamin A (DOI: 10.18632/oncotarget.9472) using Anti-Filamin A Monoclonal Antibody (FLMN01 (PM6/317)) (Product # MA5-11705) in Western Blot. {RE}

Product Details

MA5-11705

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat, Chicken, Guinea pig

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

FLMN01 (PM6/317)

Immunogen

Platelet filamin

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10982048

Product Specific Information

MA5-11705 targets Filamin in ELISA, IHC (P), and WB applications and shows reactivity with Chicken, Guinea Pig, Human, mouse, Rabbit, and Rat samples.

The MA5-11705 immunogen is platelet filamin.

Target Information

Filamin functions as a crosslinking protein forming a flexible link between two actin filaments. It is composed of two identical polypeptide chains each joined to the other at one end, with an actin binding site at the other. It is present in human platelets, lymphocytes, fibroblasts and smooth muscle actin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Unfolded Protein Response Sensor PERK Mediates Stiffness-Dependent Adaptation in Glioblastoma Cells.

International journal of molecular sciences

Khoonkari M,Liang D,Lima MT,van der Land T,Liang Y,Sun J,Dolga A,Kamperman M,van Rijn P,Kruyt FAE

Published figure using Filamin A monoclonal antibody (Product # MA5-11705) in Immunocytochemistry

Fri Jun 10 00:00:00 EDT 2022

Inducible microRNA-200c decreases motility of breast cancer cells and reduces filamin A.

PloS one

Ljepoja B,Schreiber C,Gegenfurtner FA,García-Roman J,Köhler B,Zahler S,Rädler JO,Wagner E,Roidl A

MA5-11705 was used in Western Blotting to describe two miR-200c inducible breast cancer cell lines, derived from miR-200c knock-out MCF7 cells as well as from the miR200c-negative MDA-MB-231 cells and report on the emerging phenotypic effects after miR-200s induction.

Thu Apr 02 00:00:00 EDT 2020

Inducible microRNA-200c decreases motility of breast cancer cells and reduces filamin A.

PloS one

Ljepoja B,Schreiber C,Gegenfurtner FA,García-Roman J,Köhler B,Zahler S,Rädler JO,Wagner E,Roidl A

Thu Apr 02 00:00:00 EDT 2020

Calpain 3 is activated through autolysis within the active site and lyses sarcomeric and sarcolemmal components.

Molecular and cellular biology

Taveau M,Bourg N,Sillon G,Roudaut C,Bartoli M,Richard I

MA5-11705 was used in western blot to study the mechanism of calpain-3 activation and to identify substrates in immature muscle cells

Mon Dec 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: 450 kDa protein; ABP-280; actin binding protein 280; Actin-binding protein 280; alpha filamin; Alpha-filamin; dilated pupils 2; Endothelial actin-binding protein; Endothelial actin-binding protein 280); filamin 1; filamin A, alpha; filamin, alpha; Filamin-1; Filamin-A; FilaminA; FLN-A; gizzard filamin; Non-muscle filamin

Gene Aliases: ABP-280; ABPX; CSBS; CVD1; Dilp2; F730004A14Rik; filamin-1; filamin-A; FLN; FLN-A; FLN1; FLNA; FMD; GENA 379; MNS; NHBP; OPD; OPD1; OPD2; RGD1560614; XLVD; XMVD

UniProt ID: (Human) P21333, (Mouse) Q8BTM8

Entrez Gene ID: (Rabbit) 100328754, (Chicken) 395261, (Human) 2316, (Rat) 293860, (Guinea pig) 100725162, (Mouse) 192176

Function(s)