Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis of Human Fractalkine was performed by loading 0.25µg of recombinant human Fractalkine onto a 4-20% Tris-HCl polyacrylamide gel. Protein was transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membrane was probed with a human Fractalkine monoclonal antibody (Product # MA1-031) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1% Tween-20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least one hour. Membrane was washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Non-glycosylated, polypeptide chain containing 76 amino acids of chemokine domain expressed in E. coli.|
|Storage buffer||PBS with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-031 has been successfully used in Western blot and ELISA applications with human samples.
The immunogen for MA1-031 is a non-glycosylated, polypeptide chain containing 76 amino acids of Fractalkine's chemokine domain (molecular weight of ~8638 Da) expressed in E. coli.
Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. This molecule can exist in 2 forms: as membrane-anchored or as a shed 95-kD glycoprotein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C-X3-C motif chemokine 1; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; FKN; NTT; SCYD1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small-inducible cytokine D1
A-152E5.2; ABCD-3; C3Xkine; CX3CL1; CXC3; CXC3C; FKN; fractalkine; neurotactin; NTN; NTT; SCYD1