|Tested species reactivity||Bacteria|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified F.tularensis strain 15 LPS.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21690 detects the virulent and vaccine strains of Francisella tularensis LPS from infected samples. There is no cross-reactivity with Fr. Novicida, Br. abortus, Br. suis, Br. melitensis, Br. ovis, Y. pestis, Y. enterocolitica, Y. pseudotuberculosis, E.Coli, V. cholerae, B.neotomae, or S.typhimurium.
MA1-21690 has been successfully used in ELISA and immunofluorescence procedures.
The MA1-21690 immunogen is purified F.tularensis strain 15 LPS. Spleen cells of Balb/c mice were immunised with purified F.tularensis strain 15 LPS. The epitope is located on the O-antigen polysaccharide chain.
Francisella tularensis is a small, nonmotile, aerobic, gram-negative coccobacillus. It has a thin lipopolysaccharide-containing envelope and is a hardy non-spore-forming organism that survives for weeks at low temperatures in water, moist soil, hay, straw, and decaying animal carcasses. Lipopolysaccaride (LPS) is a main species-specific antigen of Francisella tularensis. According to the data available LPS of tularemia microbe differs from LPS of other gram-negative bacteria. LPS of tularemia microbe hasn't yet revealed the properties of classical endotoxin, which may be connected with non-typical structure of lipid A, a toxic component of LPS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.