Immunofluorescent analysis of G-CSF Receptor/CD114 showing staining in the cytoplasm of THP-1 cells. THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a G-CSF Receptor/CD114 polyclonal antibody (Product # PA5-28988) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 164 and 432 of Human GCSF Receptor|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28988 targets GCSF Receptor in IF and WB applications and shows reactivity with Human samples.
The PA5-28988 immunogen is recombinant fragment corresponding to a region within amino acids 164 and 432 of Human GCSF Receptor.
The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, with three of the isoforms being membrane-bound and the other being secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.
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