|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to amino acid residues surrounding the Phospho-GABA-B (pSer892) Receptor R2 Subunit.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OPA1-04119 detects phospho GABA-B (pSer892) receptor R2 subunit in rat samples.
OPA1-04119 has been successfully used in Immunofluroescence and Western blot procedures. By Western blot, this antibody detects a ~110 kDa protein representing phospho GABA-B (pSer892) receptor R2 subunit in rat brain membrane fractions.
The OPA1-04119 immunizing peptide corresponds to the amino acid residues surrounding the phospho GABA-B (pSer892) receptor R2 subunit.
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA
receptors the GABA-A and the GABA-B subtype of receptors. GABA-B receptors are heterodimeric
G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system.
Moss and colleagues (Couve, et al., 2002) recently demonstrated that the functional coupling of
GABA(B)R1/GABA(B)R2 receptors to inwardly rectifying K+ channels rapidly desensitizes. This
effect is alleviated after direct phosphorylation of a single serine residue (Ser892) in the cytoplasmic tail of GABA(B)R2 by cyclic AMP (cAMP)-dependent protein kinase (PKA). In addition to it postsynaptic effects GABAB receptors localized to the presynaptic region have been reported to restrict the availability of synaptic vesicles for release (Sakaba and Neher, 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.