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|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein from the cytoplasmic loop of the gamma 2 subunit of rat GABRG2|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:1,000|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, chicken, canine, human, mouse and non-human primate based on 100% sequence homology.
In Western blot, this antibody detects a 44-46 kDa protein representing GABA-A receptor subunit gamma 2 from rat brain fractions.
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four ßs and four ?s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for a- and ß-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a ?-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
GABA(A) receptor subunit gamma-2; Gamma aminobutyric acid (GABA) A receptor gamma 2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid receptor subunit gamma-2