Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:1,000 | View 2 publications 2 publications |
Immunocytochemistry (ICC/IF) |
Assay-dependent | View 1 publication 1 publication |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
GAD1 Fusion Protein Ag0666 (1-224 aa encoded by BC002815) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.29 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.1% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MASSTPSSS ATSSNAGADP NTTNLRPTTY DTWCGVAHGC TRKLGLKICG FLQRTNSLEE KSRLVSAFKE RQSSKNLLSC ENSDRDARFR RTETDFSNLF ARDLLPAKNG EEQTVQFLLE VVDILLNYVR KTFDRSTKVL DFHHPHQLLE GMEGFNLELS DHPESLEQIL VDCRDTLKYG VRTGHPRFFN QLSTGLDIIG LAGEWLTSTA NTNMPSDMRE CWLLR (1-224 aa encoded by BC002815)
GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 67 kDa glutamic acid decarboxylase; FLJ45882; GAD 67; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 variant GAD67NT; Glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); glutamic acid decarboxylase 1; OTTHUMP00000041055
Gene Aliases: CPSQ1; EP10; GAD; Gad-1; GAD1; GAD25; GAD44; GAD67; SCP
UniProt ID: (Human) Q99259, (Mouse) P48318, (Rat) P18088
Entrez Gene ID: (Human) 2571, (Mouse) 14415, (Rat) 24379
Molecular Function:
decarboxylase
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support
