Abnova

GCLC Monoclonal Antibody (3H1)

Product Image Gallery

GCLC Antibody in Immunocytochemistry (ICC) — Immunofluorescence of monoclonal antibody to GCLC on HeLa cell. Antibody concentration is 10 µg/mL.

GCLC Antibody in Western Blot (WB) — Western Blot analysis of GCLC expression in transfected 293T cell line by GCLC monoclonal antibody (M01), clone 3H1.

Lane 1: GCLC transfected lysate(72.8 KDa).
Lane 2: Non-transfected lysate.

GCLC Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged GCLC is approximately 0.03 ng/mL as a capture antibody.

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.03 ng/mL

Immunofluorescence (IF)

10 µg/mL

Western Blot (WB)

1-5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3H1

Immunogen

GCLC (NP_001489, 528 a.a. approximately 637 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: EGVFPGLIPI LNSYLENMEV DVDTRCSILN YLKLIKKRAS GELMTVARWM REFIANHPDY KQDSVITDEM NYSLILKCNQ IANELCECPE LLGSAFRKVK YSGSKTDSSN

Target Information

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this antibody

Bioinformatics

Protein Aliases: gamma GCS-HS; Gamma-ECS; Gamma-glutamylcysteine synthetase; gamma-glutamylcysteine synthetase heavy subunit; GCS heavy chain; Glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit; Glutamylcysteine gamma synthetase light chain

Gene Aliases: D9Wsu168e; GCL; GCLC; GCS; Ggcs-hs; GLCL; GLCL-H; GLCLC

UniProt ID: (Human) P48506, (Rat) P19468, (Mouse) P97494

Entrez Gene ID: (Human) 2729, (Rat) 25283, (Mouse) 14629

Molecular Function: ligase