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Immunofluorescent analysis of GFAP (green) showing staining in the in the cytoplasm of SK-N-MC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GFAP monoclonal antibody (Product # MA5-12023) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Chicken, Human, Pig, Rat|
|Published species reactivity||Rat, Pig, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Glial Fibrillary Acidic Protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12023 targets Glial Fibrillary Acidic Protein in IF, IHC (P), and WB applications and shows reactivity with Chicken, Human, Porcine, and Rat samples.
The MA5-12023 immunogen is glial Fibrillary Acidic Protein.
Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Ab-6 stains astrocytes, glial cells, ependymal cells and their corresponding tumors. Many types of neural tumors such as neuroblastomas, Schwannomas, as well as extra-CNS tumors are not labeled.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Enhancement of Neural Stem Cells after Induction of Depression in Male Albino Rats (A histological & Immunohistochemical Study).
MA5-12023 was used in immunohistochemistry - paraffin section to test if rhodiola rosea extract stimulates neuronal stem cell proliferation and improves depression
|Kamel Ismail ZM,Morcos MA,Eldin Mohammad MD,Gamal Aboulkhair A||International journal of stem cells (7:70)||2014|
Multipotent adult hippocampal progenitor cells maintained as neurospheres favor differentiation toward glial lineages.
MA5-12023 was used in immunocytochemistry and immunohistochemistry to study the preference for glial differentiation of multipotent adult hippocampal progenitors cells cultured as free-floating cellular aggregates
|Oh J,Daniels GJ,Chiou LS,Ye EA,Jeong YS,Sakaguchi DS||Biotechnology journal (9:921)||2014|
Electroacupuncture promotes proliferation of amplifying neural progenitors and preserves quiescent neural progenitors from apoptosis to alleviate depressive-like and anxiety-like behaviours.
MA5-12023 was used in immunohistochemistry to study the beneficial effects of electroacupuncture in a rat model of depression and the role of proliferation of amplifying neural progenitors and inhibition of quiescent neural progenitor apoptosis
|Yang L,Yue N,Zhu X,Han Q,Li B,Liu Q,Wu G,Yu J||Evidence-based complementary and alternative medicine : eCAM (2014:null)||2014|
Changes in retinal aquaporin-9 (AQP9) expression in glaucoma.
MA5-12023 was used in immunohistochemistry to investigate the expression of retinal aquaporin-9 in glaucoma
|Yang MH,Dibas A,Tyan YC||Bioscience reports (33:null)||2014|
T cell-activation in neuromyelitis optica lesions plays a role in their formation.
MA5-12023 was used in immunohistochemistry to study the role of T-cell activation in the formation of neuromyelitis optica lesions
|Pohl M,Kawakami N,Kitic M,Bauer J,Martins R,Fischer MT,Machado-Santos J,Mader S,Ellwart JW,Misu T,Fujihara K,Wekerle H,Reindl M,Lassmann H,Bradl M||Acta neuropathologica communications (1:null)||2014|
Fractalkine signaling and Tau hyper-phosphorylation are associated with autophagic alterations in lentiviral Tau and Aβ1-42 gene transfer models.
MA5-12023 was used in immunohistochemistry to study changes in autophagy and the roles of Tau hyperphosphorylation and fractalkine in an animal model of Abeta42-induced Tau hyperphosphorylation
|Hebron ML,Algarzae NK,Lonskaya I,Moussa C||Experimental neurology (251:127)||2014|
Involvement of the spinal NALP1 inflammasome in neuropathic pain and aspirin-triggered-15-epi-lipoxin A4 induced analgesia.
MA5-12023 was used in immunohistochemistry to study the role of reduced NALP1 inflammasome activation in the analgesic effects of 15-epilipoxin A4 in a rat model of neuropathic pain
|Li Q,Tian Y,Wang ZF,Liu SB,Mi WL,Ma HJ,Wu GC,Wang J,Yu J,Wang YQ||Neuroscience (254:230)||2013|
NDGA reduces secondary damage after spinal cord injury in rats via anti-inflammatory effects.
MA5-12023 was used in immunohistochemistry to study the role of anti-inflammatory mechanisms in ability of nordihydroguaiaretic acid to protect against seconday damage in a rat model of spinal cord injury
|Xue H,Zhang XY,Liu JM,Song Y,Liu TT,Chen D||Brain research (1516:83)||2013|
Intrathecal lamotrigine attenuates mechanical allodynia and suppresses microglial and astrocytic activation in a rat model of spinal nerve ligation.
MA5-12023 was used in immunohistochemistry to study the beneficial effects on mechanical allodynia and on the activation of microglia and astrocytes of intrathecal administration of lamotrigine in a rat model of neuropathic pain
|Choi YS,Jun IG,Kim SH,Park JY||Yonsei medical journal (54:321)||2013|
Development of a chemically extracted acellular muscle scaffold seeded with amniotic epithelial cells to promote spinal cord repair.
MA5-12023 was used in immunohistochemistry to develop a novel acellular muscle scaffold containing amniotic epithelial cells for the repair of spinal cord injury
|Xue H,Zhang XY,Liu JM,Song Y,Li YF,Chen D||Journal of biomedical materials research. Part A (101:145)||2013|
Conditional Müllercell ablation causes independent neuronal and vascular pathologies in a novel transgenic model.
MA5-12023 was used in immunohistochemistry to study the neural and vascular pathologies resulting from the conditional ablation of Mller cells in a novel murine model
|Shen W,Fruttiger M,Zhu L,Chung SH,Barnett NL,Kirk JK,Lee S,Coorey NJ,Killingsworth M,Sherman LS,Gillies MC||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:15715)||2012|
Activation of Src family kinases in spinal microglia contributes to formalin-induced persistent pain state through p38 pathway.
MA5-12023 was used in immunohistochemistry to study the role of spinal microglial Src and p38 MAPK in persistant pain in a formalin injection animal model
|Tan YH,Li K,Chen XY,Cao Y,Light AR,Fu KY||The journal of pain : official journal of the American Pain Society (13:1008)||2012|
Expression pattern of ataxia telangiectasia mutated (ATM), p53, Akt, and glycogen synthase kinase-3β in the striatum of rats treated with 3-nitropropionic acid.
MA5-12023 was used in immunohistochemistry to study the immunohistochemical expression of ATM, p53, Akt and GSK3-beta in a rat model of neurodegeneration
|Duran-Vilaregut J,Manich G,Del Valle J,Camins A,Pallàs M,Vilaplana J,Pelegrí C||Journal of neuroscience research (90:1803)||2012|
The correlation between DTI parameters and levels of AQP-4 in the early phases of cerebral edema after hypoxic-ischemic/reperfusion injury in piglets.
MA5-12023 was used in immunohistochemistry to use a porcine model of hypoxic ischemia-reperfusion injury to study the relationship between diffusion tenser imaging parameters and aquaporin-4 expression in early-stage cerebral edema
|Wang H,Wang X,Guo Q||Pediatric radiology (42:992)||2012|
Differentiated adipose-derived stem cells promote myelination and enhance functional recovery in a rat model of chronic denervation.
MA5-12023 was used in immunohistochemistry to study the axon myelination and functional recovery promoted by differentiated adipose-derived stem cells in a chronically denervated rat model
|Tomita K,Madura T,Mantovani C,Terenghi G||Journal of neuroscience research (90:1392)||2012|
Genetic ablation of Pals1 in retinal progenitor cells models the retinal pathology of Leber congenital amaurosis.
MA5-12023 was used in immunohistochemistry to develop a murine model of Leber congenital amaurosis by deleting retinal progenitor cell Pals1
|Cho SH,Kim JY,Simons DL,Song JY,Le JH,Swindell EC,Jamrich M,Wu SM,Kim S||Human molecular genetics (21:2663)||2012|
NADPH oxidase expression in active multiple sclerosis lesions in relation to oxidative tissue damage and mitochondrial injury.
MA5-12023 was used in immunohistochemistry to study the role of NADPH oxidase in oxidative tissue damage and mitochondrial injury in active multiple sclerosis lesions
|Fischer MT,Sharma R,Lim JL,Haider L,Frischer JM,Drexhage J,Mahad D,Bradl M,van Horssen J,Lassmann H||Brain : a journal of neurology (135:886)||2012|
Changes in lipid-sensitive two-pore domain potassium channel TREK-1 expression and its involvement in astrogliosis following cerebral ischemia in rats.
MA5-12023 was used in immunocytochemistry and immunohistochemistry to study the effects of cerebral ischemia on the expression of TREK-1 and the significance for astrogliosis
|Wang M,Song J,Xiao W,Yang L,Yuan J,Wang W,Yu Z,Xie M||Journal of molecular neuroscience : MN (46:384)||2012|
CD8+ T-cell clones dominate brain infiltrates in Rasmussen encephalitis and persist in the periphery.
MA5-12023 was used in immunohistochemistry to examine the involvement of CD8+ T cells in the pathogenesis of Rasmussen encephalitis
|Schwab N,Bien CG,Waschbisch A,Becker A,Vince GH,Dornmair K,Wiendl H||Brain : a journal of neurology (132:1236)||2009|
Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver.
MA5-12023 was used in immunohistochemistry to study ether xenotransplantation of pig fetal liver fragments can provide better growth and proliferation compared with isolated pig fetal hepatoblasts
|Katchman H,Tal O,Eventov-Friedman S,Shezen E,Aronovich A,Tchorsh D,Cohen S,Shtabsky A,Hecht G,Dekel B,Freud E,Reisner Y||Stem cells (Dayton, Ohio) (26:1347)||2008|
TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain.
MA5-12023 was used in immunocytochemistry, immunohistochemistry, and western blot to study the different pathological effects of mutant TDP-43 in murine neuronal and glial cells
|Yan S,Wang CE,Wei W,Gaertig MA,Lai L,Li S,Li XJ||Human molecular genetics (23:2678)||2014|
Cdh1 inhibits reactive astrocyte proliferation after oxygen-glucose deprivation and reperfusion.
MA5-12023 was used in immunocytochemistry to study the proliferation of reactive astrocytes following glucose/oxygen deprivation-reperfusion and the inhibitory effect of Cdh1
|Qiu J,Zhang C,Lv Y,Zhang Y,Zhu C,Wang X,Yao W||Neurochemistry international (63:87)||2013|
Involvement of TREK-1 activity in astrocyte function and neuroprotection under simulated ischemia conditions.
MA5-12023 was used in immunocytochemistry to study the role TREK-1 in the response of astrocytes to ischemia and the therapeutic significance
|Wu X,Liu Y,Chen X,Sun Q,Tang R,Wang W,Yu Z,Xie M||Journal of molecular neuroscience : MN (49:499)||2013|
Adsorption of mesenchymal stem cells and cortical neural stem cells on carbon nanotube/polycarbonate urethane.
MA5-12023 was used in immunocytochemistry to study the effect of carbon nanotubes on stem cell survival
|Nho Y,Kim JY,Khang D,Webster TJ,Lee JE||Nanomedicine (London, England) (5:409)||2010|
The multifaceted effects of agmatine on functional recovery after spinal cord injury through Modulations of BMP-2/4/7 expressions in neurons and glial cells.
MA5-12023 was used in immunohistochemistry and western blot to study the roles of neuronal and glial BMP-2, -4 and -7 in the benefecial effects of agmatine on recovery from spinal cord injury in a murine model
|Park YM,Lee WT,Bokara KK,Seo SK,Park SH,Kim JH,Yenari MA,Park KA,Lee JE||PloS one (8:null)||2013|
glial fibrillary acidic protein; glial fibrillary acidic protein alpha; intermediate filament