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|Tested species reactivity||Tag|
|Host / Isotype||Mouse / IgG1|
|Immunogen||The GFP was isolated directly from the jellyfish Aequorea victoria.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A11121 is optimized for Western analysis, allowing colorimetric detection of as little as 10 ng of GFP or GFP-fusion proteins, or chemiluminescent detection of picogram quantities.
Storage and reconstitution: to prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibodies in 0.5 mL PBS, pH 7.4. Store solution for up to 3 months at 4°C with the addition of 2mM sodium azide.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Green Fluorescence Protein