|Tested species reactivity||Bovine, Hamster, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgM|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 130-143 of GJA1.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:200-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:500|
|Western Blot (WB)||1:8000-1:16,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25097 detects GJA1 from human, mouse, rat, bovine, and hamster samples.
MA1-25097 has been successfully used in ELISA, immunohistochemistry (frozen), immunohistochemistry (paraffin), and Western blot procedures.
The MA1-25097 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 130-143 of GJA1.
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.