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Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:1,000 | - |
Immunohistochemistry (IHC) |
1:50-1:200 | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Recombinant Monoclonal |
Type |
Antibody |
Clone |
JF09-08 |
Immunogen |
Synthetic peptide within Human Gli1 aa 38-82 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
Contains |
0.05% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2809830 |
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GLI-Kruppel family member GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; Glioma-associated oncogene homolog; glioma-associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; zinc finger protein 5; Zinc finger protein GLI1
Gene Aliases: AV235269; GLI; GLI1; Zfp-5; Zfp5
UniProt ID: (Human) P08151, (Mouse) P47806
Entrez Gene ID: (Human) 2735, (Rat) 140589, (Mouse) 14632
Molecular Function:
DNA-binding transcription factor
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