Invitrogen

GLO1 Monoclonal Antibody (Glo1a)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

5 References

View all (26) GLO1 antibodies

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Protocols

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GLO1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on cancer biopsies of deparaffinized Human Prostate and Stomach cancer tissues. To expose target proteins high pressure heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Following antigen retrieval endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes at room-temp. Tissues were then washed in PBS and blocked in 10% normal goat serum for 20 minutes at room temperature. Tissues were probed at a dilution of 1:800 with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBS. Colorimetric detection was performed using metal enhanced DAB and tissues counterstained with hematoxylin. Images are displayed at 40X magnification. Results demonstrate cytoplasmic localization of Glyoxylase.

GLO1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), MDA-MB-231 (Lane 3), HL-60 (Lane 4), MOLT-4 (Lane 5), LNCaP (Lane 6), T-47D (Lane 7), U-87 MG (Lane 8) and A549 (Lane 9). The blot was probed with Mouse Anti-GLO1 Monoclonal Antibody (Product # MA1-13029, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 21 kDa band corresponding to GLO1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

GLO1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. THP-1 cells were transfected with GLO1 siRNA and decrease in signal intensity was observed in western blot application using GLO1 Monoclonal Antibody (Product # MA1-13029X).. {KD}

Product Details

MA1-13029

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 4 publications 4 publications
Immunohistochemistry (IHC)	1:800	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Published species	Human, Rat
Host/Isotype	Mouse / IgG
Class	Monoclonal
Type	Antibody
Clone	Glo1a
Immunogen	Recombinant human GLOI-GST fusion protein.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_11154644

Target Information

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of Glyoxalase-I Leads to Reduced Proliferation, Migration and Colony Formation, and Enhanced Susceptibility to Sorafenib in Hepatocellular Carcinoma.

Frontiers in oncology

Michel M,Hollenbach M,Pohl S,Ripoll C,Zipprich A

MA1-13029 was used in Immunohistochemistry to analyse Glo-I in hepatocellular carcinoma and the effect of the multi-tyrosine kinase inhibitor sorafenib on Glo-I.

Thu Oct 01 00:00:00 EDT 2020

Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice.

Cells

Emidio GD,Placidi M,Rea F,Rossi G,Falone S,Cristiano L,Nottola S,D'Alessandro AM,Amicarelli F,Palmerini MG,Tatone C

Published figure using GLO1 monoclonal antibody (Product # MA1-13029) in Western Blot

Tue Jan 14 00:00:00 EST 2020

Glycine increases glyoxalase-1 function by promoting nuclear factor erythroid 2-related factor 2 translocation into the nucleus of kidney cells of streptozotocin-induced diabetic rats.

Journal of diabetes investigation

Wang Z,Zhao D,Chen L,Li J,Yuan G,Yang G,Zhang H,Guo X,Zhang J

MA1-13029 was used in Western Blotting to determine the role of glyoxalase-1 and activation of the nuclear factor erythroid 2-related factor 2 in the mitigation of oxidative stress in rat kidneys.

Sun Sep 01 00:00:00 EDT 2019

Glycine Suppresses AGE/RAGE Signaling Pathway and Subsequent Oxidative Stress by Restoring Glo1 Function in the Aorta of Diabetic Rats and in HUVECs.

Oxidative medicine and cellular longevity

Wang Z,Zhang J,Chen L,Li J,Zhang H,Guo X

MA1-13029 was used in Western Blotting to evaluate whether glycine can attenuate oxidative stress by suppressing the AGE/RAGE signalling pathway in the aorta of streptozotocin-induced diabetic rats and in human umbilical vascular endothelial cells.

Tue Apr 23 00:00:00 EDT 2019

Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction.

Nature medicine

Qi W,Keenan HA,Li Q,Ishikado A,Kannt A,Sadowski T,Yorek MA,Wu IH,Lockhart S,Coppey LJ,Pfenninger A,Liew CW,Qiang G,Burkart AM,Hastings S,Pober D,Cahill C,Niewczas MA,Israelsen WJ,Tinsley L,Stillman IE,Amenta PS,Feener EP,Vander Heiden MG,Stanton RC,King GL

MA113029 was used in western blot to study the role of pyruvate kinase M2 in diabetic nephropathy

Thu Jun 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Aldoketomutase; epididymis secretory protein Li 74; Glx I; glyoxalase 1 complex; glyoxalase 1 regulatory; glyoxalase 1 structural; glyoxalase domain containing 1; Glyoxalase I; glyoxylase 1; Ketone-aldehyde mutase; lactoyl glutathione lyase; Lactoylglutathione lyase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase

Gene Aliases: 0610009E22Rik; 1110008E19Rik; 2510049H23Rik; AW550643; Glo-1; Glo-1r; Glo-1s; GLO1; Glo1-r; Glo1-s; GLOD1; GLY1; GLYI; HEL-S-74; Qglo

UniProt ID: (Human) Q96J41, (Rat) Q6P7Q4, (Mouse) Q9CPU0

Entrez Gene ID: (Human) 2739, (Rat) 294320, (Mouse) 109801

Molecular Function: lyase