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Immunohistochemistry analysis of GLUT-1 showing staining in the cytoplasm of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GLUT-1 Mouse Monoclonal Antibody (MA511315) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||A synthetic peptide derived from C-terminal of human GLUT-1|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11315 targets GLUT-1 in IHC (P) applications and shows reactivity with Human and Rat samples.
The MA5-11315 immunogen is a synthetic peptide derived from C-terminal of human GLUT-1.
Glucose is fundamental to the metabolism in mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast. GLUT-1 immunoreactivity in some cancers, including trans carcinoma of the urinary bladder, has been associated with aggressive behavior.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HIF-1α and CA-IX as predictors of locoregional control for determining the optimal treatment modality for early-stage laryngeal carcinoma.
MA5-11315 was used in immunohistochemistry to study the value of the immunohistochemical expression of HIF-1alpha and carbonic anhydrase IX for selecting the optimal treatment strategy in patients with early-stage laryngeal carcinoma
|Kwon OJ,Park JJ,Ko GH,Seo JH,Jeong BK,Kang KM,Woo SH,Kim JP,Hwa JS,Carey TE||Head & neck (37:505)||2015|
High-risk human papillomavirus load and biomarkers in cervical intraepithelial neoplasia and cancer.
MA5-11315 was used in immunohistochemistry to study the relationships between high risk HPV load, the expression of various biomarkers, and cervical intraepithelial neoplasia and cancer
|Chang MS,Oh S,Jung EJ,Park JH,Jeon HW,Lee TS,Kim JH,Choi E,Byeon SJ,Park IA||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (122:427)||2014|
High FDG activity in focal fat necrosis: a pitfall in interpretation of posttreatment PET/CT in patients with non-Hodgkin lymphoma.
MA5-11315 was used in immunohistochemistry to study the ability of focal fat necrosis to confound interpretation of PET/CT data in non-Hodgkin lymphoma patients
|Kashyap R,Lau E,George A,Seymour JF,Lade S,Hicks RJ,Hofman MS||European journal of nuclear medicine and molecular imaging (40:1330)||2013|
Prognostic assessment of hypoxia and metabolic markers in cervical cancer using automated digital image analysis of immunohistochemistry.
MA5-11315 was used in immunohistochemistry to study the prognostic value of automated image analysis of the immunohistochemical expression of hypoxic and metabolic markers in cervical cancer patients
|Kim BW,Cho H,Chung JY,Conway C,Ylaya K,Kim JH,Hewitt SM||Journal of translational medicine (11:null)||2013|
Tenascin-C, GLUT-1, and syndecan-2 expression in juvenile nasopharyngeal angiofibroma: correlations to vessel density and tumor stage.
MA5-11315 was used in immunohistochemistry to study the expression of tenascin-C, Glut-1 and syndecan-2 in juvenile nasopharyngeal angiofibroma and their potential role in angiogenesis and growth
|Renkonen S,Heikkilä P,Haglund C,Mäkitie AA,Hagström J||Head & neck (35:1036)||2013|
Transforming growth factor-β1 may be a key mediator of the fibrogenic properties of neural cells in leprosy.
MA5-11315 was used in immunohistochemistry to study the role of TGF-beta1 in driving Schwann cell phenotypic reprogramming and fibrogenesis in response to Mycobacterium leprae
|Petito RB,Amadeu TP,Pascarelli BM,Jardim MR,Vital RT,Antunes SL,Sarno EN||Journal of neuropathology and experimental neurology (72:351)||2013|
IMP3 and GLUT-1 immunohistochemistry for distinguishing benign from malignant mesothelial proliferations.
MA5-11315 was used in immunohistochemistry to study the diagnostic value of IMP3 and Glut1 in benign and malignant mesothelial proliferations
|Lee AF,Gown AM,Churg A||The American journal of surgical pathology (37:421)||2013|
Neuropilin-2 and vascular endothelial growth factor receptor-3 are up-regulated in human vascular malformations.
MA5-11315 was used in immunohistochemistry to study the expression of different VEGF isoforms and their receptors in 33 human vascular anomalies
|Partanen TA,Vuola P,Jauhiainen S,Lohi J,Salminen P,Pitkäranta A,Häkkinen SK,Honkonen K,Alitalo K,Ylä-Herttuala S||Angiogenesis (16:137)||2013|
Down-regulation of MutS homolog 3 by hypoxia in human colorectal cancer.
MA5-11315 was used in immunohistochemistry to study the role of HIF1-alpha in the mechanism by which human colon cancer MSH3 is downregulated in response to hypoxia
|Li J,Koike J,Kugoh H,Arita M,Ohhira T,Kikuchi Y,Funahashi K,Takamatsu K,Boland CR,Koi M,Hemmi H||Biochimica et biophysica acta (1823:889)||2012|
Carcinoma ex pleomorphic adenoma of the salivary glands: distinct clinicopathologic features and immunoprofiles between subgroups according to cellular differentiation.
MA5-11315 was used in immunohistochemistry to study the clinicopathological implications and mechanisms of cellular differentiation in carcinoma ex pleomorphic adenoma
|Kim JW,Kwon GY,Roh JL,Choi SH,Nam SY,Kim SY,Cho KJ||Journal of Korean medical science (26:1277)||2011|
αB-crystallin: a novel regulator of breast cancer metastasis to the brain.
MA5-11315 was used in immunocytochemistry to study the role of alphaB-crystallin in modulating triple-negative breast cancer brain metastasis
|Malin D,Strekalova E,Petrovic V,Deal AM,Al Ahmad A,Adamo B,Miller CR,Ugolkov A,Livasy C,Fritchie K,Hamilton E,Blackwell K,Geradts J,Ewend M,Carey L,Shusta EV,Anders CK,Cryns VL||Clinical cancer research : an official journal of the American Association for Cancer Research (20:56)||2014|
choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); erythrocyte/brain; facilitated glucose transporter member 1; glucose transporter type 1; glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1; hepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; receptor for HTLV-1 and HTLV-2; solute carrier family 2; solute carrier family 2 (facilitated glucose transporter), member 1; Solute carrier family 2 a 1 (facilitated glucose transporter) brain; solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, member 1
CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; GLUTB; GTG1; Gtg3; HTLVR; PED; RATGTG1; SDCHCN; SLC2A1