|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from internal region of human GRSF-1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is brain tissue.
G-rich sequence factor 1 (GRSF-1) is a cytoplasmic protein that binds RNAs containing the G-rich element. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. GRSF-1 is up-regulated with prostate cancer progression and down regulated by the differentiating agents phenylacetate and phenylbutyrate. The function of GRSF-1 is uncharacterized, but potential roles include mRNA transport, stabilization, or translation. It might react with G-rich containing mRNA of Bcl2 and Bax indicating a regulation role of GRSF-1 in metastatic prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: G-rich sequence factor 1; GRSF-1
Gene Aliases: GRSF1
UniProt ID: (Human) Q12849
Entrez Gene ID: (Human) 2926
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