Invitrogen

GSC Monoclonal Antibody (4C5D5)

View all (17) GSC antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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GSC Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GSC in paraffin-embedded liver cancer tissue. Sample was incubated with GSC monoclonal antibody (Product # MA5-31756) using a dilution of 1:200-1:1000 followed by DAB staining.

GSC Antibody in Western Blot (WB) — Western blot analysis of GSC in HEK293 (1) and GSC cell lysate. Samples were incubated with GSC monoclonal antibody (Product # MA5-31756) using a dilution of 1:500-1:2000.

GSC Antibody in Flow Cytometry (Flow) — Flow cytometry of GSC in HeLa cells (green). Sample was incubated with GSC monoclonal antibody (Product # MA5-31756) using a dilution of 1:200-1:400 followed by negative control (red).

GSC Antibody in ELISA (ELISA) — ELISA analysis of GSC in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with GSC monoclonal antibody (Product # MA5-31756) using a dilution of 1:10,000.

Product Details

MA5-31756

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4C5D5

Immunogen

Purified recombinant fragment of human GSC (AA: 191-257) expressed in E. coli.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2787379

Product Specific Information

MA5-31756 has been tested in indirect ELISA.

Target Information

This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Homeobox protein goosecoid

Gene Aliases: GSC; SAMS

UniProt ID: (Human) P56915

Entrez Gene ID: (Human) 145258

Molecular Function: homeodomain transcription factor

Function(s)

negative regulation of transcription from RNA polymerase II promoter gastrulation neural crest cell fate specification dorsal/ventral neural tube patterning signal transduction involved in regulation of gene expression negative regulation of Wnt signaling pathway forebrain development middle ear morphogenesis muscle organ morphogenesis embryonic skeletal system morphogenesis

Process(es)

RNA polymerase II transcription factor binding RNA polymerase II repressing transcription factor binding sequence-specific DNA binding