|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human protein purified from E.coli (His-GSK3B)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is A431 cells.
Glycogen synthase kinase-3beta (GSK-3beta) is a cytoplasmic serine/threonine protein kinase, which was initially identified as the kinase that phosphorylates glycogen synthase to inhibit glycogen synthesis. GSK3 beta is a ubiquitously expressed kinase that regulates a wide variety of cellular functions including metabolism, gene expression and cytoskeletal integrity. Akt/PI3K reduces GSK-3beta activity by phosphorylation of its Ser9 residue. GSK-3 beta has a critical role in the regulation of the amount of cyclin D1, as this kinase is involved in both cyclin D1 mRNA transcription and ubiquitin-dependent proteolysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.