|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region of human GTF2H1.|
|Storage buffer||PBS with 2% sucrose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||4-8 µg/ml|
|Western Blot (WB)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24494 detects GTF2H1 in Human samples.
PA1-24494 has been successfully used in Immunohistochemistry and Western Blot procedures.
PA1-24494 immunogen corresponds synthetic peptide corresponding to a region of human GTF2H1.
TFIIH, a multisubunit complex is involved in several biological fundamental mechanisms of the cell: transcription, nucleotide excision repair and cell cycle regulation. p62 is one of the six subunits that constitutes the core of TFIIH. Analysis of the expression of the p62 gene reveals an over expression in testis tissue. This subunit of TFIIH participates in a variety of protein protein interactions. For example, Rb competes with TBP and p62 for binding to E2F thus repressing E2F mediated transactivation; herpes simplex virus VP16 and human p53, directly interact with the p62 subunit of TFIIH. In addition, TFIIH, via p62 phosphorylation is the major target for mitotic inactivation of transcription. GTF2H1 is a component of the core GTF2H basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. GTF2H1 is one of the six subunits forming the core GTF2H basal transcription factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.