Immunohistochemical analysis of Glutamic Acid Decarboxylase 67 using anti-Glutamic Acid Decarboxylase 67 Polyclonal Antibody (PA5-32431) in Pancreas Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from near N-terminus of mouse GAD67 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is pancreas tissue.
Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to gamma-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. GAD67 is a cytoplasmic protein and is encodes on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.