Immunofluorescent analysis of Glutathione S-transferase kappa 1 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a Glutathione S-transferase kappa 1 polyclonal antibody (Product # PA5-30918) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 21 and 226 of Human Glutathione S-transferase kappa 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30918 targets Glutathione S-transferase kappa 1 in IHC (P), ICC/IF, and WB applications and shows reactivity with Human samples.
The PA5-30918 immunogen is recombinant fragment corresponding to a region within amino acids 21 and 226 of Human Glutathione S-transferase kappa 1.
This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.