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Human IgM Secondary Antibody (SA5-10293) in ELISA
ELISA analysis was performed using Human IgM antigen (500 ng/well, 60 min). Samples were blocked for 60 min with 1% BSA, and incubated with HRP Goat anti-Human IgM (Product # SA5-10293) for 60 min. Development was done with TMB substrate (10 min) and the reaction was stopped with 0.3M H2SO4. Results were read on a microplate reader at OD450.
Product Details
SA5-10293
Applications
Tested Dilution
Western Blot (WB)
Assay-dependent
Immunohistochemistry (IHC)
Assay-dependent
ELISA (ELISA)
Assay-dependent
Immunoelectrophoresis (IE)
Assay-dependent
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Human IgM
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
50mM HEPES with proprietary stabilizer
Contains
0.002% thimerosal
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_2868341
Target
IgM
Antibody Form
Whole Antibody
Product Specific Information
This antibody may cross react with IgM from other species.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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