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HeLa cells. Anti–human Golgin-97, mouse monoclonal CDF4, Alexa Fluor® 430 goat anti–mouse IgG, Alexa Fluor® 594 phalloidin, and SYTOX® Green nucleic acid stain. Fixed, permeabilized and RNase-treated HeLa cells were labeled with mouse monoclonal anti–human Golgin-97 monoclonal antibody (Cat. no. A21270) and visualized with the yellow-fluorescent Alexa Fluor® 430 goat anti–mouse IgG antibody (Cat. no. A11063). The F-actin was labeled with red-fluorescent Alexa Fluor® 594 phalloidin (Cat. no. A12381) and the nuclei were stained with the SYTOX® Green nucleic acid stain (Cat. no. S7020). This multiple-exposure image was acquired using a Nikon Diaphot fluorescent microscope equipped with a Quantix CCD camera.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 430|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Immunofluorescence technique for 100-nm-thick semithin sections of Epon-embedded tissues.||Haraguchi CM,Yokota S||Histochemistry and cell biology (117:81)||2002|