The yellow-green fluorescent Alexa Fluor 514 dye is far superior to fluorescein in both brightness and photostability, and it can be detected with standard fluorescein, Oregon Green dye or Alexa Fluor 488 dye filter sets. By using instruments that collect spectral distribution information, the signal from the Alexa Fluor 514 dye can be distinguished from both the Alexa Fluor 488 and Alexa Fluor 500 dyes.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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