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|Tested species reactivity||Rabbit|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.4, with 0.1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Immunoblot (IB)||0.2-10 µg/mL|
|Immunomicroscopy (IM)||0.2-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The NANOGOLD conjugate of Fab' fragment of goat anti-rabbit IgG is labeled with small uniformly sized (1.4 nm) gold particles at a ~1:1 ratio in the hinge region, remote from the antigen-binding site and can be used in immunoblotting, light microscopy, and electron microscopy applications.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.