Immunofluorescent analysis of Golgi protein in HeLa cells. Cells were fixed and permeabilized with 3% PFA plus 0.05% Triton X-100 in PBS and stained using a Golgi protein, 58k monoclonal antibody (Product # MA1-22144) at a dilution of 1:50, followed by detection using an anti-Mouse IgG conjugated to Cy3 at a dilution of 1:100.
|Tested species reactivity||Dog, Hamster, Human, Pig, Rat, Bovine|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, full length, native, rat Golgi protein from rat liver.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunomicroscopy (IM)||Assay dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-22144 detects golgi protein, 58K in human, rat, canine, and hamster samples. This antibody is expected to cross react with porcine and bovine Golgi protein due to sequence homology.
MA1-22144 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), immunomicroscopy and Western blot procedures.
The MA1-22144 immunogen is purified, full length, native, rat Golgi protein from rat liver.
Store at 4°C for short term. For extended storage, aliquot and store at -20°C, Avoid Freeze/Thaw Cycles.
58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
G protein ß¿ subunits regulate cardiomyocyte hypertrophy through a perinuclear Golgi phosphatidylinositol 4-phosphate hydrolysis pathway.
MA1-22144 was used in immunocytochemistry to characterize cardiomyocyte hypertrophy and regulation by G protein beta-gamma subunits through perinuclear Golgi phosphatidylinositol 4-phosphate hydrolysis pathway
|Malik S,deRubio RG,Trembley M,Irannejad R,Wedegaertner PB,Smrcka AV||Molecular biology of the cell (26:1188)||2015|
Mitochondrial dysfunction driven by the LRRK2-mediated pathway is associated with loss of Purkinje cells and motor coordination deficits in diabetic rat model.
MA1-22144 was used in western blot to determine how leucine-rich repeat kinase 2 contributes to diabetic neuropathy using Purkinje cells
|Yang S,Xia C,Li S,Du L,Zhang L,Hu Y||Cell death and disease (5:null)||2014|