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Immunofluorescent analysis of Golgi protein in HeLa cells. Cells were fixed and permeabilized with 3% PFA plus 0.05% Triton X-100 in PBS and stained using a Golgi protein, 58k monoclonal antibody (Product # MA1-22144) at a dilution of 1:50, followed by detection using an anti-Mouse IgG conjugated to Cy3 at a dilution of 1:100.
|Tested species reactivity||Rat, Bovine, Dog, Hamster, Human, Pig|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, full length, native, rat Golgi protein from rat liver.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunomicroscopy (IM)||Assay dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-22144 detects golgi protein, 58K in human, rat, canine, and hamster samples. This antibody is expected to cross react with porcine and bovine Golgi protein due to sequence homology.
MA1-22144 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), immunomicroscopy and Western blot procedures.
The MA1-22144 immunogen is purified, full length, native, rat Golgi protein from rat liver.
Store at 4°C for short term. For extended storage, aliquot and store at -20°C, Avoid Freeze/Thaw Cycles.
58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mitochondrial dysfunction driven by the LRRK2-mediated pathway is associated with loss of Purkinje cells and motor coordination deficits in diabetic rat model.
MA1-22144 was used in western blot to determine how leucine-rich repeat kinase 2 contributes to diabetic neuropathy using Purkinje cells
|Yang S,Xia C,Li S,Du L,Zhang L,Hu Y||Cell death & disease (5:null)||2014|
58 kDa microtubule-binding protein; formimidoyltransferase cyclodeaminase; Formimidoyltransferase-cyclodeaminase; formiminotransferase cyclodeaminase; formiminotransferase-cyclodeaminase
FTCD; I79_006831; LCHC1