Immunohistochemical analysis of Golgi protein, 58k in paraffin-embedded human liver tissue using a Golgi protein, 58k polyclonal antibody (Product # PA1-31351) at a dilution of 3.8 µg/ml. Steamed antigen retrieval was performed using a citrate buffer, pH 6, followed by AP-staining.
|Tested species reactivity||Human, Mouse, Pig|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide SQLVECVPNFSEGKNQ, corresponding to N terminal amino acids 2-17 of Human 58K Golgi protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||3-5 ug/ml|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31351 detects 58K Golgi protein from human samples.
PA1-31351 has been successfully used in Western blot applications.
The PA1-31351 immunogen is: Synthetic peptide: SQLVECVPNFSEGKNQ, corresponding to N terminal amino acids 2-17 of Human 58K Golgi protein.
The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.
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