|Tested species reactivity||Bovine, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified recombinant human Grb2 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA5-12165 targets Grb2 in IF and IHC (P) applications and shows reactivity with Bovine, Human, mouse, Rat, and Xenopus laevis samples.
The MA5-12165 immunogen is purified recombinant human Grb2 protein.
Grb2 protein contains one SH2 domain flanked on both sides by an SH3 domain. Ab-1 binds to the junction between the amino terminal SH3 domain and the SH2 domain of Grb2. Reactivity with Grb2 deletion mutants suggests that its epitope maps between aa 54-62. In response to EGF, Grb2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The Grb2-Sos complex then binds the EGF-receptor. These interactions play a critical role in the activation of the p21ras signalling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Endosomal targeting of MEK2 requires RAF, MEK kinase activity and clathrin-dependent endocytosis.
MA5-12165 was used in immunocytochemistry to investigate the molecular mechanism for the endosomal targeting of MEK2
|Galperin E,Sorkin A||Traffic (Copenhagen, Denmark) (9:1776)||2008|