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|Tested species reactivity||Tag|
|Published species reactivity||Dog, C. elegans, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||A synthetic peptide corresponding to the nine amino acid sequence HA-tag (YPYDVPDYA).|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ZO proteins redundantly regulate the transcription factor DbpA/ZONAB.
32-6700 was used in western blot to study the role of ZO proteins in the regulation of transcription factor DbpA/ZONAB
|Spadaro D,Tapia R,Jond L,Sudol M,Fanning AS,Citi S||The Journal of biological chemistry (289:22500)||2014|
|C. elegans||1:500||Isoforms of the polarity protein par6 have distinct functions.||Gao L,Macara IG||The Journal of biological chemistry (279:41557)||2004|
|Human||Not Cited||Cbl-dependent ubiquitination is required for progression of EGF receptors into clathrin-coated pits.||Stang E,Blystad FD,Kazazic M,Bertelsen V,Brodahl T,Raiborg C,Stenmark H,Madshus IH||Molecular biology of the cell (15:3591)||2004|