Immunofluorescent analysis of HDAC2 in HeLa cells, fixed and permeabilized with cold methanol followed by cold methanol:acetone, using a HDAC2 monoclonal antibody (Product # MA1-25451) at a dilution of 10 µg/mL followed by detection using a Goat anti-Mouse IgG, Cy3 conjugate.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues C-S(471) G E K T D T K G T K S E Q L S N P(488) of human HDAC2, with an N-terminal added cystine.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||0.25-0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25451 detects HDAC2 from human, mouse, rat, bovine, and canine samples.
MA1-25451 has been successfully used in immunoprecipitation, immunohistochemistry, ELISA, and Western blot procedures.
The MA1-25451 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues C-S(471) G E K T D T K G T K S E Q L S N P(488) of human HDAC2, with an N-terminal added cysteine.
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.