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|Tested species reactivity||Virus|
|Host / Isotype||Mouse / IgG1|
|Clone||15 (Tat 15)|
|Immunogen||Recombinant Tat protein of HIV-1 BH10 purified from E. coli|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-3 µl|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts HIV-1 Tat of BH10 and IIIB strains, subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE). It has been mapped to amino acids 2-9 (EPVDPRLE - B subtype a.a. consensus).
MA1-71510 can be used for chromatin immunoprecipitation (ChIP) analysis of HIV1 Tat.
HIV-1 Tat ("Trans-Activator of Transcription") consists of 86 to 101 amino acids depending on the subtype. Tat is critical to HIV reproduction by way of compensating for a defect in its genome: the HIV RNA initially has a hairpin-structured portion which prevents full transcription from occurring. However, a small number of RNA transcripts will be made, which allow the Tat protein to be produced. Tat then binds to and phosphorylates cellular factors, eliminating the effect of the hairpin RNA structure and allowing transcription of the HIV RNA. This increases the rate of transcription, providing a positive feedback cycle. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HIV-1 Tat region 2-9; Human immunodeficiency virus